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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-12-23
|
| pubmed:abstractText |
Prosaposin, the precursor of sphingolipid activator protein (saposins A-D), has been identified as a neurotrophic factor capable of inducing neural differentiation and preventing cell death. The putative prosaposin receptor was partially purified from baboon brain membranes by affinity chromatography using a saposin C-column. The purified preparation gave a single major protein band with an apparent molecular weight of 54 kDa on SDS-PAGE. Affinity cross-linking of 11 kDa 125I-saposin C demonstrated the presence of a 66 kDa product, indicative of an apparent molecular weight of 55 kDa for the receptor. A GTP gamma S-binding assay using cell membranes from SHSY5Y neural cells demonstrated agonist stimulated binding of [35S]-GTP gamma S upon treatment with prosaptide TX14(A) a peptide from the neurotrophic region; maximal binding was obtained at 2 nM. TX14(A) stimulated binding was abolished by prior treatment of SHSY5Y cells with pertussis toxin and by a scrambled and an all D-amino acid-derivative of the 14-mer. A 14-mer mutant prosaptide (6N-->6D) competed with TX14(A) with a Ki of 0.7 nM. Immunoblot analysis using an antibody against the G0 alpha subunit demonstrated that the purified receptor preparation contained a 40 kDa reactive band consistent with association of G0 alpha and the receptor. These findings indicate that the signaling induced by prosaposin and TX14(A) is generated by binding to a G0-protein associated receptor.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Psap protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Saposins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-291X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
17
|
| pubmed:volume |
240
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
415-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9388493-Amino Acid Sequence,
pubmed-meshheading:9388493-Animals,
pubmed-meshheading:9388493-Brain,
pubmed-meshheading:9388493-Cell Membrane,
pubmed-meshheading:9388493-Chromatography, Affinity,
pubmed-meshheading:9388493-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9388493-GTP-Binding Proteins,
pubmed-meshheading:9388493-Glycoproteins,
pubmed-meshheading:9388493-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:9388493-Mice,
pubmed-meshheading:9388493-Molecular Weight,
pubmed-meshheading:9388493-Nerve Growth Factors,
pubmed-meshheading:9388493-Neuroblastoma,
pubmed-meshheading:9388493-Papio,
pubmed-meshheading:9388493-Peptide Fragments,
pubmed-meshheading:9388493-Receptors, Cell Surface,
pubmed-meshheading:9388493-Receptors, G-Protein-Coupled,
pubmed-meshheading:9388493-Saposins,
pubmed-meshheading:9388493-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Prosaposin receptor: evidence for a G-protein-associated receptor.
|
| pubmed:affiliation |
Department of Neurosciences, University of California, School of Medicine, San Diego, La Jolla 92093-0634, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|