Linked Life Data

9388261

Source:http://linkedlifedata.com/resource/pubmed/id/9388261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
49
pubmed:dateCreated
1998-1-8
pubmed:abstractText
Human monocyte chemoattractant protein-1 (human MCP-1) mRNA accumulated in THP-1 cells 2 h after lipopolysaccharide (LPS) stimulation. DNase I footprinting revealed that LPS stimulation induced protein binding to the two closely located NF-kappaB sites, A1 and A2. By electrophoretic gel mobility shift assay and supershift assay, the binding of (p65)2, c-Rel/p65, p50/p65, and p50/c-Rel to the A2 oligonucleotide probe was detected after LPS stimulation. In contrast, 12-o-tetradecanoylphorbol 13-acetate did not induce a significant amount of MCP-1 mRNA in THP-1 cells 2 h after stimulation, and only p50/p65 bound to the A2 probe. trans-Activity of each NF-kappaB/Rel dimer was investigated by transfecting P19 cells with p65, p50, and/or c-Rel expression vectors, and a luciferase construct containing the enhancer region of the human MCP-1 gene. Expression of recombinant p65 or p65 and c-Rel resulted in elevated luciferase activities, indicating that (p65)2 and c-Rel/p65 had trans-activity. The binding of (p65)2 and/or c-Rel/p65 to the A2 probe was also detected from 12-o-tetradecanoylphorbol 13-acetate-stimulated HeLa, HOS, and A172 cells in which expression of MCP-1 mRNA was elevated. Finally, the role of the A1 site was investigated. Both (p65)2 and c-Rel/p65 bound to the A1 probe by electrophoretic mobility shift assay and a mutation in the A1 or A2 site resulted in a loss of the enhancer activity. These results suggest that the binding of (p65)2 and c-Rel/p65 to the A1 and A2 sites of this gene is important for the tissue- and stimulus-specific transcription of the human MCP-1 gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
272
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31092-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9388261-Base Sequence, pubmed-meshheading:9388261-Binding Sites, pubmed-meshheading:9388261-Cell Line, pubmed-meshheading:9388261-Chemokine CCL2, pubmed-meshheading:9388261-DNA, pubmed-meshheading:9388261-DNA Footprinting, pubmed-meshheading:9388261-Dimerization, pubmed-meshheading:9388261-Gene Expression Regulation, pubmed-meshheading:9388261-HeLa Cells, pubmed-meshheading:9388261-Humans, pubmed-meshheading:9388261-Lipopolysaccharides, pubmed-meshheading:9388261-Molecular Sequence Data, pubmed-meshheading:9388261-NF-kappa B, pubmed-meshheading:9388261-NF-kappa B p50 Subunit, pubmed-meshheading:9388261-Protein Binding, pubmed-meshheading:9388261-Protein Conformation, pubmed-meshheading:9388261-RNA, Messenger, pubmed-meshheading:9388261-Sequence Homology, Nucleic Acid, pubmed-meshheading:9388261-Tetradecanoylphorbol Acetate, pubmed-meshheading:9388261-Transcription, Genetic, pubmed-meshheading:9388261-Transcription Factor RelA
pubmed:year
1997
pubmed:articleTitle
Transcriptional regulation of the human monocyte chemoattractant protein-1 gene. Cooperation of two NF-kappaB sites and NF-kappaB/Rel subunit specificity.
pubmed:affiliation
Immunopathology Section, Laboratory of Immunobiology, National Cancer Institute-Frederick, Cancer Research and Development Center, Frederick, Maryland 21702, USA.
pubmed:publicationType
Journal Article