Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-12-16
pubmed:abstractText
When the 513 bp amplified fragments of HBV S-gene, known subtypes, were digested with Msp I and BamH I, the following phenomena appeared: adr subtypic 513 bp was fragmentized to 320 bp and 193 bp by Msp I; ayw subtype was digested and fragmentized to 295 bp and 218 bp by BamH I; ayw could also be fragmentized to become 326 bp and 187 bp digested by Msp I. Howerev, adw subtype was not digested by Msp I and BamH I nor adr by BamH I. The experimental patterns in agarose gel electrophoresis were tallied with the expected patterns. Each HBV subtypes gave unique size of digestive fragments which was easy to observe and distinguish on agarose gel. This study provided a new classification scheme for HBV-DNA and could be used for epidemiological investigation of HBV infection.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0254-6450
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
356-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
[A simplified method for detection of genomic subtyps of HBV S-gene].
pubmed:affiliation
Centre of Molecular Biology, Shenzhen People's Hospital.
pubmed:publicationType
Journal Article, English Abstract