Linked Life Data

9385375

Source:http://linkedlifedata.com/resource/pubmed/id/9385375

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-12-24
pubmed:abstractText
In this paper we present a new application of the detection of nuclear transcripts by fluorescence in situ hybridization (FISH) for studying the transcriptional activity of amplified genes in tumour cells. As a model, we have used the A431 cell line in which several amplification sites have been identified. We focused on two amplified regions: (1) the 6p12 region, which was found amplified by using comparative genomic hybridization, and which contains an amplification of the hsp90 beta gene; (2) the 7p12-p13 region, which displays a 20- to 30-fold amplification of the gene encoding the epidermal growth factor receptor (EGFr). By using FISH to detect nuclear transcripts, we show that the extra-copies of the hsp90 beta and EGFr genes are actively transcribed within the sites of amplification. This work illustrates the potential of this method as a tool for functional in situ cytogenetic analyses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0340-6717
pubmed:author
pubmed:issnType
Print
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Analysis of the transcriptional activity of amplified genes in tumour cells by fluorescence in situ hybridization.
pubmed:affiliation
Inserm U309, Equipe DyOGen, Institut Albert Bonniot, Université Joseph Fourier Grenoble I, La Tronche, France. caroline.jolly@ujf-grenoble.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't