Linked Life Data

9383042

Source:http://linkedlifedata.com/resource/pubmed/id/9383042

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-12-22
pubmed:abstractText
Many studies have demonstrated that astrocytes respond with fluctuations in intracellular calcium concentration ([Ca2+]i) and membrane potential following the application of a number of ligands. Moreover, calcium (Ca2+) waves that spread through astrocytic syncitia have been described in numerous reports. We had the rare opportunity to study Ca2+ responses in astrocytes obtained from a patient diagnosed with Rasmussen's encephalitis, a rare form of intractable epilepsy. Using the ratiometric fluorescent indicator fura-2, we observed large spontaneous [Ca2+]i oscillations. The mean time between initial rise in [Ca2+]i and the return to baseline was 5.1 +/- 0.19 minutes (SEM; n = 201) and [Ca2+]i increased to a mean level of 271 +/- 8 nM (SEM; n = 201) from a baseline of 136 +/- 6 nM (SEM; n = 201). Removal of Ca2+ from the perfusion solution combined with the addition of the Ca2+ chelator EGTA (2 mM) completely but reversibly eliminated all oscillations suggesting the fluctuations were dependent on Ca2+ flux across the membrane. The percentage of cells undergoing spontaneous changes in [Ca2+]i decreased over time in culture. At 10-11 days postsurgery, approximately 70% of the cells were exhibiting this behavior, and by day 23 transients were no longer observed. We did not observe comparable spontaneous [Ca2+]i oscillations in rat cortical astrocytes. The potential that the spontaneous [Ca2+]i oscillations observed may be a unique feature of epileptic tissues is discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0894-1491
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
332-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Spontaneous intracellular calcium oscillations in cortical astrocytes from a patient with intractable childhood epilepsy (Rasmussen's encephalitis).
pubmed:affiliation
Department of Neurobiology, University of Alabama at Birmingham, 35294, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.