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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1998-1-30
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pubmed:abstractText |
Current therapy for several forms of anemia involves a weekly regime of multiple subcutaneous injections of recombinant human erythropoietin (hEpo). In an effort to provide a physiologically regulated administration of erythropoietin, we are developing cell lines genetically engineered to release hEpo as a function of oxygen tension. C2C12 cells were transfected using a vector containing the hEpo cDNA driven by the hypoxia-responsive promoter to the murine phosphoglycerate kinase gene. In vitro, these cells showed a threefold increase in hEpo secretion as oxygen levels were shifted from 21% to 1.3% oxygen. To test in vivo response, C2C12-hEpo cells were encapsulated in a microporous membrane and implanted subcutaneously on the dorsal flank of DBA/2J mice. On average, serum hEpo levels in animals exposed to 7% oxygen were two-fold higher than values seen in their control counterparts kept at 21% oxygen. Similar studies employing rats confirmed that hEpo delivery is regulated as a function of oxygen tension. These results suggest the feasibility of developing an oxygen-regulated, encapsulated cell-based system for hEpo delivery.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Hif1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Hif1a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoxia-Inducible Factor 1, alpha...,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoglycerate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1043-0342
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1881-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9382954-Anemia,
pubmed-meshheading:9382954-Animals,
pubmed-meshheading:9382954-Anoxia,
pubmed-meshheading:9382954-Cells, Cultured,
pubmed-meshheading:9382954-DNA-Binding Proteins,
pubmed-meshheading:9382954-Drug Compounding,
pubmed-meshheading:9382954-Erythropoietin,
pubmed-meshheading:9382954-Gene Expression Regulation,
pubmed-meshheading:9382954-Gene Therapy,
pubmed-meshheading:9382954-Histocytochemistry,
pubmed-meshheading:9382954-Hypoxia-Inducible Factor 1,
pubmed-meshheading:9382954-Hypoxia-Inducible Factor 1, alpha Subunit,
pubmed-meshheading:9382954-Mice,
pubmed-meshheading:9382954-Mice, Inbred DBA,
pubmed-meshheading:9382954-Nuclear Proteins,
pubmed-meshheading:9382954-Oxygen,
pubmed-meshheading:9382954-Partial Pressure,
pubmed-meshheading:9382954-Phosphoglycerate Kinase,
pubmed-meshheading:9382954-Plasmids,
pubmed-meshheading:9382954-Promoter Regions, Genetic,
pubmed-meshheading:9382954-Rats,
pubmed-meshheading:9382954-Rats, Inbred F344,
pubmed-meshheading:9382954-Recombinant Proteins,
pubmed-meshheading:9382954-Transcription Factors,
pubmed-meshheading:9382954-Transgenes
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pubmed:year |
1997
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pubmed:articleTitle |
A gene therapy approach to regulated delivery of erythropoietin as a function of oxygen tension.
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pubmed:affiliation |
Gene Therapy Center, Centre Hospitalier Universitaire Vaudois, Lausanne University Medical School, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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