Linked Life Data

9380759

Source:http://linkedlifedata.com/resource/pubmed/id/9380759

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1997-11-10
pubmed:abstractText
Applying a brief repolarizing pre-pulse to a depolarized frog skeletal muscle fiber restores a small fraction of the transverse tubule membrane voltage sensors from the inactivated state. During a subsequent depolarizing test pulse we detected brief, highly localized elevations of myoplasmic Ca2+ concentration (Ca2+ "sparks") initiated by restored voltage sensors in individual triads at all test pulse voltages. The latency histogram of these events gives the gating pattern of the sarcoplasmic reticulum (SR) calcium release channels controlled by the restored voltage sensors. Both event frequency and clustering of events near the start of the test pulse increase with test pulse depolarization. The macroscopic SR calcium release waveform, obtained from the spark latency histogram and the estimated open time of the channel or channels underlying a spark, exhibits an early peak and rapid marked decline during large depolarizations. For smaller depolarizations, the release waveform exhibits a smaller peak and a slower decline. However, the mean use time and mean amplitude of the individual sparks are quite similar at all test depolarizations and at all times during a given depolarization, indicating that the channel open times and conductances underlying sparks are essentially independent of voltage. Thus, the voltage dependence of SR Ca2+ release is due to changes in the frequency and pattern of occurrence of individual, voltage-independent, discrete release events.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-1082506, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-1082510, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-13714849, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-2213590, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-2855645, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-6606034, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-6608964, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-6655593, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-7516645, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-7569901, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-7754383, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8010748, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8010750, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8235594, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8537819, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8559251, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8889203, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-8968567, http://linkedlifedata.com/resource/pubmed/commentcorrection/9380759-9003545
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11061-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Voltage dependence of the pattern and frequency of discrete Ca2+ release events after brief repriming in frog skeletal muscle.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD 21201, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't