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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1997-11-10
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pubmed:abstractText |
The suprachiasmatic nucleus (SCN), a circadian oscillator, contains various peptides arranged in the compartment-specific manner. Somatostatin (SS) and substance P (SP), a peptide derived from preprotachykinin A (PPT-A), are expressed in neurons in the intermediate zone, a narrow area between the major dorsomedial and ventrolateral subdivisions. In the present study, we examined the possibility of co-localization of SS and SP in the SCN by a double-labeling in situ hybridization method using 35S-labeled and digoxigenin-labeled cRNA probes. In the SCN, most of preprosomatostatin (PPSS) mRNA-containing neurons expressed PPT-A mRNA (86%) and, in turn, almost all preprotachykinin A (PPT-A) mRNA-expressing neurons expressed PPSS mRNA signals (97%). Both PPSS and PPT-A mRNAs were also detected in the cerebral cortex and the caudate-putamen, however, their co-existence was extremely rare (< 4%) in these regions. Since the pharmacological effects of SS and SP are similar to that of the light pulses exposed on animals under constant darkness, the co-release of peptides might be an important process for entraining the circadian clock in the SCN.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin,
http://linkedlifedata.com/resource/pubmed/chemical/Substance P,
http://linkedlifedata.com/resource/pubmed/chemical/Tachykinins,
http://linkedlifedata.com/resource/pubmed/chemical/preprotachykinin
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0169-328X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9379838-Animals,
pubmed-meshheading:9379838-Caudate Nucleus,
pubmed-meshheading:9379838-Cerebral Cortex,
pubmed-meshheading:9379838-In Situ Hybridization,
pubmed-meshheading:9379838-Male,
pubmed-meshheading:9379838-Neurons,
pubmed-meshheading:9379838-Organ Specificity,
pubmed-meshheading:9379838-Protein Precursors,
pubmed-meshheading:9379838-Putamen,
pubmed-meshheading:9379838-RNA, Messenger,
pubmed-meshheading:9379838-Rats,
pubmed-meshheading:9379838-Rats, Wistar,
pubmed-meshheading:9379838-Somatostatin,
pubmed-meshheading:9379838-Substance P,
pubmed-meshheading:9379838-Suprachiasmatic Nucleus,
pubmed-meshheading:9379838-Tachykinins,
pubmed-meshheading:9379838-Transcription, Genetic
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pubmed:year |
1997
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pubmed:articleTitle |
Co-localization of preprosomatostatin mRNA and preprotachykinin A mRNA in neurons of the rat suprachiasmatic nucleus.
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pubmed:affiliation |
Department of Anatomy and Brain Science, Kobe University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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