Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
20
|
| pubmed:dateCreated |
1997-11-10
|
| pubmed:abstractText |
Members of cutaneous melanoma (CM) families with dysplastic nevi (DN) are at high risk of developing CM. Using a shuttle vector plasmid, pSP189, cell lines from three patients with CM plus DN were previously found to have elevated post-UV plasmid mutability. To investigate familial occurrence of this cellular phenotype, we examined post-UV plasmid mutability in 31 lymphoblastoid cell lines from 6 familial CM kindreds. In comparison to 16 normal control lines, we found an abnormally elevated post-UV plasmid mutability in cell lines from 13 of 13 patients with CM plus DN (P = 1.5 x 10(-8)) and from 5 of 8 patients with DN only (P = 0.001). Elevated spontaneous plasmid mutation frequency (MF) was also present in cell lines from six of the CM plus DN patients (P = 0.002) and three of the DN-only patients (P = 0.028). However, cell lines from two patients with CM without DN had normal post-UV plasmid MF. Although not specific for CM patients, of 27 cell lines with elevated post-UV plasmid MF, only 8 were from donors who did not have CM + DN or DN (19 of 24 versus 8 of 28; P = 0.0003). This study indicates that post-UV plasmid hypermutability is a laboratory marker for members of melanoma-prone families and suggests that patients with familial CM have a defective mechanism for handling UV-induced DNA damage.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0008-5472
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4637-41
|
| pubmed:dateRevised |
2008-8-12
|
| pubmed:meshHeading |
pubmed-meshheading:9377580-Age Factors,
pubmed-meshheading:9377580-Cell Line, Transformed,
pubmed-meshheading:9377580-DNA Repair,
pubmed-meshheading:9377580-Dysplastic Nevus Syndrome,
pubmed-meshheading:9377580-Family,
pubmed-meshheading:9377580-Genetic Vectors,
pubmed-meshheading:9377580-Herpesvirus 4, Human,
pubmed-meshheading:9377580-Humans,
pubmed-meshheading:9377580-Lymphocytes,
pubmed-meshheading:9377580-Melanoma,
pubmed-meshheading:9377580-Mutagenesis,
pubmed-meshheading:9377580-Mutagenicity Tests,
pubmed-meshheading:9377580-Plasmids,
pubmed-meshheading:9377580-Skin Neoplasms,
pubmed-meshheading:9377580-Tumor Cells, Cultured,
pubmed-meshheading:9377580-Ultraviolet Rays,
pubmed-meshheading:9377580-Xeroderma Pigmentosum
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Hypermutability of UV-treated plasmids in dysplastic nevus/familial melanoma cell lines.
|
| pubmed:affiliation |
Laboratory of Molecular Carcinogenesis, National Cancer Institute, Bethesda, Maryland 20892, USA.
|
| pubmed:publicationType |
Journal Article
|