| pubmed-article:9375956 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C1565860 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0038925 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0017710 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C2936832 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C1412362 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:9375956 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:9375956 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9375956 | pubmed:dateCreated | 1998-1-2 | lld:pubmed |
| pubmed-article:9375956 | pubmed:abstractText | 1. The objective of the present study was to determine the effects of dexamethasone on key constituents of prostaglandin and leukotriene biosynthesis, cyclo-oxygenase-2 (COX-2) and 5-lipoxygenase activating protein (FLAP). The human monocytic cell line THP-1 was used as a model system. mRNA and protein levels of COX-2 and FLAP were determined by Northern and Western blot analyses, respectively. 2. Low levels of COX-2 and FLAP mRNA were expressed in undifferentiated THP-1 cells, but were induced upon differentiation of the cells along the monocytic pathway by treatment with phorbol ester (TPA, 5 nM). Maximal expression was observed after two days. 3. Coincubation of the undifferentiated cells with dexamethasone (10(-9) - 10(-6) M) and phorbol ester prevented induction of COX-2 mRNA, but did not affect the induction of FLAP mRNA. 4. Dexamethasone downregulated COX-2 mRNA and protein in differentiated, monocyte-like THP-1 cells. In contrast, FLAP mRNA and protein were upregulated by dexamethasone in differentiated THP-1 cells. After 24 h, FLAP mRNA levels were increased more than 2 fold. Dexamethasone did not change 5-lipoxygenase mRNA expression. 5. Release of prostaglandin E2 (PGE2) and peptidoleukotrienes was determined in cell culture supernatants of differentiated THP-1 cells by ELISA. Calcium ionophore-dependent PGE2 synthesis was associated with COX-2 expression, whereas COX-1 and COX-2 seemed to participate in arachidonic acid-dependent PGE2 synthesis. Very low levels of peptidoleukotrienes were released from differentiated THP-1 cells upon incubation with ionophore. Treatment with dexamethasone did not significantly affect leukotriene release. 6. These data provide evidence that prostaglandin synthesis is consistently downregulated by glucocorticoids. However, the glucocorticoid-mediated induction of FLAP may provide a mechanism to maintain leukotriene biosynthesis through more efficient transfer of arachidonic acid to the 5-lipoxygenase reaction, in spite of inhibitory effects on other enzymes of the biosynthetic pathway. | lld:pubmed |
| pubmed-article:9375956 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9375956 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9375956 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9375956 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:9375956 | pubmed:issn | 0007-1188 | lld:pubmed |
| pubmed-article:9375956 | pubmed:author | pubmed-author:HabenichtA... | lld:pubmed |
| pubmed-article:9375956 | pubmed:author | pubmed-author:Goppelt-Strue... | lld:pubmed |
| pubmed-article:9375956 | pubmed:author | pubmed-author:SchaeferDD | lld:pubmed |
| pubmed-article:9375956 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9375956 | pubmed:volume | 122 | lld:pubmed |
| pubmed-article:9375956 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9375956 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9375956 | pubmed:pagination | 619-24 | lld:pubmed |
| pubmed-article:9375956 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:meshHeading | pubmed-meshheading:9375956-... | lld:pubmed |
| pubmed-article:9375956 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9375956 | pubmed:articleTitle | Differential regulation of cyclo-oxygenase-2 and 5-lipoxygenase-activating protein (FLAP) expression by glucocorticoids in monocytic cells. | lld:pubmed |
| pubmed-article:9375956 | pubmed:affiliation | Department of Internal Medicine IV, University of Erlangen-Nürnberg, Erlangen, Germany. | lld:pubmed |
| pubmed-article:9375956 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9375956 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9375956 | lld:pubmed |
