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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1997-12-9
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pubmed:abstractText |
The majority of cases involving fragile X syndrome are due to expansion of a (CGG)n trinucleotide repeat at the 5' untranslated region of the FMR-1 gene. Deletion and intragenic loss of function mutations of the FMR-1 gene also have been reported. Here, we report a C to T point mutation at the 14th nucleotide in intron 10 of the FMR-1 gene in three unrelated fragile X patients. However, the (CGG)n repeat of FMR-1 in those patients does not expand. To determine the effect of this mutation on the patients' FMR-1 transcripts, total RNA from peripheral blood cells was reverse transcribed and amplified by polymerase chain reaction (RT-PCR). Direct and subcloned sequencing of the RT-PCR products revealed that the transcripts from the allele with C to T mutation skip exon 10 entirely, resulting in a joining of exons 9 and 11. Deletion of exon 10 results in frame-shift and premature termination of translation, which removes the highly conserved region that encoding the KH2 and RGG box domains of FMRP. Interestingly, a male of the three patients has another G to A substitution in exon 15. However, the intron 10 mutation is sufficient for development of fragile X syndrome.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/FMR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fragile X Mental Retardation Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins
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pubmed:status |
MEDLINE
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pubmed:issn |
1059-7794
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
393-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9375856-Adolescent,
pubmed-meshheading:9375856-Alternative Splicing,
pubmed-meshheading:9375856-Base Sequence,
pubmed-meshheading:9375856-Child,
pubmed-meshheading:9375856-DNA, Complementary,
pubmed-meshheading:9375856-Fragile X Mental Retardation Protein,
pubmed-meshheading:9375856-Fragile X Syndrome,
pubmed-meshheading:9375856-Humans,
pubmed-meshheading:9375856-Introns,
pubmed-meshheading:9375856-Male,
pubmed-meshheading:9375856-Nerve Tissue Proteins,
pubmed-meshheading:9375856-Point Mutation,
pubmed-meshheading:9375856-Polymerase Chain Reaction,
pubmed-meshheading:9375856-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:9375856-RNA-Binding Proteins,
pubmed-meshheading:9375856-Trinucleotide Repeats
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pubmed:year |
1997
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pubmed:articleTitle |
Novel point mutation within intron 10 of FMR-1 gene causing fragile X syndrome.
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pubmed:affiliation |
Molecular Genetics and Cytogenetics Laboratory, Chung Shan Medical and Dental College, Taichung, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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