Linked Life Data

9375672

Source:http://linkedlifedata.com/resource/pubmed/id/9375672

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-12-12
pubmed:abstractText
Antipeptide antibodies against the kappa-opioid receptor were used to test whether acute or chronic exposure to kappa agonists altered the phosphorylation state of the kappa-opioid receptor. Immunoprecipitation of the kappa receptor from guinea pig hippocampal slices preincubated in [32P]orthophosphoric acid revealed a basal phosphorylation of the kappa-opioid receptor. The amount of 32P incorporation into the receptor was increased following a 75-min treatment with the kappa agonist U50,488H. This effect was blocked by the selective kappa receptor antagonist norbinaltorphimine. The time course of this change in the phosphorylation state of the receptor correlated with a desensitization of the electrophysiological response to kappa agonists measured in the dentate gyrus of hippocampal slices. The phosphorylation state of the kappa-opioid receptor was also elevated in brain slices from guinea pigs made tolerant to U50,488H by 5 days of continuous exposure and then maintained in kappa agonist to avoid acute opiate withdrawal. The results of this study show that the kappa-opioid receptor was phosphorylated in an agonist-dependent manner in brain slices taken from untreated and U50,488H-tolerant animals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2405-12
pubmed:dateRevised
2011-1-19
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Agonist-induced phosphorylation of the kappa-opioid receptor.
pubmed:affiliation
Neurobiology Graduate Program, University of Washington, Seattle 98195-7280, U.S.A.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.