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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5 Pt 2
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pubmed:dateCreated |
1997-12-16
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pubmed:abstractText |
This study aimed to examine the behavior of stimulated leukocytes in the pulmonary microcirculation. The leukocyte-endothelium interaction was visualized under physiological shear rates in perfused rat lungs using high-speed confocal laser video microscopy. Leukocytes labeled with carboxyfluorescein were stimulated with cytokine-induced neutrophil chemoattractant (CINC/gro), which caused L-selectin shedding and inverse upregulation of CD18. Neither unstimulated nor stimulated leukocytes exhibited rolling in either pulmonary arterioles or venules, whereas both were sequestered in capillaries. Approximately 50% of stimulated leukocytes showed a transient cessation of movement in pulmonary capillaries. The CINC/ gro stimulation, which inhibited leukocyte rolling and adhesion to mesenteric venules, reduced leukocyte velocity and increased leukocytes in pulmonary capillaries. Pretreatment with monoclonal antibodies against intercellular adhesion molecule-1 (ICAM-1) or CD18 attenuated these changes. Confocal microfluorography revealed constitutive expression of ICAM-1 not only in venules but also abundantly in capillary networks. These results suggest that selectin-independent, CD18-ICAM-1-dependent capillary sequestration is one of the major mechanisms by which activated leukocytes accumulate in the lungs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9513
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pubmed:author |
pubmed-author:AokiTT,
pubmed-author:IigouYY,
pubmed-author:IshimuraYY,
pubmed-author:MiyataAA,
pubmed-author:NishioKK,
pubmed-author:SerizawaHH,
pubmed-author:SuematsuMM,
pubmed-author:SuzukiKK,
pubmed-author:SuzukiYY,
pubmed-author:TsumuraHH,
pubmed-author:YamaguchiKK
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pubmed:issnType |
Print
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H2361-71
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9374773-Animals,
pubmed-meshheading:9374773-Antibodies,
pubmed-meshheading:9374773-Antigens, CD18,
pubmed-meshheading:9374773-Arterioles,
pubmed-meshheading:9374773-Capillaries,
pubmed-meshheading:9374773-Chemokine CXCL1,
pubmed-meshheading:9374773-Chemokines,
pubmed-meshheading:9374773-Chemokines, CXC,
pubmed-meshheading:9374773-Chemotactic Factors,
pubmed-meshheading:9374773-Chemotaxis, Leukocyte,
pubmed-meshheading:9374773-Endothelium, Vascular,
pubmed-meshheading:9374773-Erythrocytes,
pubmed-meshheading:9374773-Growth Substances,
pubmed-meshheading:9374773-Intercellular Adhesion Molecule-1,
pubmed-meshheading:9374773-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9374773-Interleukin-8,
pubmed-meshheading:9374773-L-Selectin,
pubmed-meshheading:9374773-Male,
pubmed-meshheading:9374773-Microscopy, Video,
pubmed-meshheading:9374773-Pulmonary Alveoli,
pubmed-meshheading:9374773-Pulmonary Circulation,
pubmed-meshheading:9374773-Rats,
pubmed-meshheading:9374773-Rats, Sprague-Dawley,
pubmed-meshheading:9374773-Venules
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pubmed:year |
1997
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pubmed:articleTitle |
Role of CD18-ICAM-1 in the entrapment of stimulated leukocytes in alveolar capillaries of perfused rat lungs.
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pubmed:affiliation |
Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan.
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pubmed:publicationType |
Journal Article
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