Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1997-12-23
|
| pubmed:abstractText |
Intratumoral injection of an adenoviral vector containing radiation-inducible DNA sequences of the early growth response gene (Egr-1) promoter ligated to a cDNA encoding tumor necrosis factor-alpha (TNF-alpha; Ad.Egr-TNF) increases the radiation killing of a human radioresistant xenograft (SQ-20B). Viral dose-escalation experiments demonstrated that SQ-20B growth inhibition correlated with viral titer. Injection of 5 x 10(8) PFU Ad.Egr-TNF produced regression to a mean volume of 22 +/- 13% of the original tumor volume, 1 x 10(8) PFU to a mean of 62 +/- 24%, and 5 x 10(7) PFU to a mean of 67 +/- 27%. No regression was observed when tumors were injected with 1 x 10(7) PFU Ad.Egr-TNF or with the null viral vector (Ad.null). When two injections of vector (2 x 10(8) PFU Ad.Egr-TNF) were combined with 50 Gy, a significant increase in tumor regression was observed compared with injection of buffer, Ad.Egr-TNF, or 50 Gy. The interactive killing between TNF and radiation was enhanced significantly (P = 0.05) when the number of injections was increased from two to five while maintaining a constant viral titer (2 x 10(8) PFU Ad.Egr-TNF) and a constant radiation dose (50 Gy). Significant TNF-alpha levels were present in irradiated vs. unirradiated tumors following injection with Ad.Egr-TNF. Taken together, these data suggest that the volumetric reduction produced by the combined effects of Ad.Egr-TNF and radiation is enhanced with increasing vector concentration and the number of vector injections.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EGR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Egr1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1065-7541
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
5
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
220-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9372544-Adenoviridae,
pubmed-meshheading:9372544-Animals,
pubmed-meshheading:9372544-Carcinoma, Squamous Cell,
pubmed-meshheading:9372544-Combined Modality Therapy,
pubmed-meshheading:9372544-DNA-Binding Proteins,
pubmed-meshheading:9372544-Early Growth Response Protein 1,
pubmed-meshheading:9372544-Female,
pubmed-meshheading:9372544-Gene Therapy,
pubmed-meshheading:9372544-Genetic Vectors,
pubmed-meshheading:9372544-Humans,
pubmed-meshheading:9372544-Immediate-Early Proteins,
pubmed-meshheading:9372544-Injections, Intralesional,
pubmed-meshheading:9372544-Mice,
pubmed-meshheading:9372544-Mice, Nude,
pubmed-meshheading:9372544-Neoplasm Transplantation,
pubmed-meshheading:9372544-Transcription Factors,
pubmed-meshheading:9372544-Transplantation, Heterologous,
pubmed-meshheading:9372544-Tumor Necrosis Factor-alpha,
pubmed-meshheading:9372544-Zinc Fingers
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Increased injection number enhances adenoviral genetic radiotherapy.
|
| pubmed:affiliation |
Department of Radiation and Cellular Oncology, Pritzker School of Medicine, University of Chicago, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|