Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1998-1-8
pubmed:databankReference
pubmed:abstractText
(E)-beta-Farnesene is a sesquiterpene semiochemical that is used extensively by both plants and insects for communication. This acyclic olefin is found in the essential oil of peppermint (Mentha x piperita) and can be synthesized from farnesyl diphosphate by a cell-free extract of peppermint secretory gland cells. A cDNA from peppermint encoding (E)-beta-farnesene synthase was cloned by random sequencing of an oil gland library and was expressed in Escherichia coli. The corresponding synthase has a deduced size of 63.8 kDa and requires a divalent cation for catalysis (Km for Mg2+ approximately 150 microM; Km for Mn2+ approximately 7 microM). The sesquiterpenoids produced by the recombinant enzyme, as determined by radio-GC and GC-MS, are (E)-beta-farnesene (85%), (Z)-beta-farnesene (8%), and delta-cadinene (5%) with the native C15 substrate farnesyl diphosphate (Km approximately 0.6 microM; Vrel = 100) and Mg2+ as cofactor, and (E)-beta-farnesene (98%) and (Z)-beta-farnesene (2%) with Mn2+ as cofactor (Vrel = 80). With the C10 analog, GDP, as substrate (Km = 1.5 microM; Vrel = 3 with Mg2+ as cofactor), the monoterpenes limonene (48%), terpinolene (15%), and myrcene (15%) are produced.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-11607499, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-1273595, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-1400496, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-1438319, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-1495034, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-1595887, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-2298278, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-2441623, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-4121143, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-7706281, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8053674, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8078910, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8117108, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8226816, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8554317, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8621577, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-8798524, http://linkedlifedata.com/resource/pubmed/commentcorrection/9371761-9268308
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12833-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-beta-farnesene.
pubmed:affiliation
Institute of Biological Chemistry, and Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-6340, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't