| pubmed-article:9371698 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C1335671 | lld:lifeskim |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C0033413 | lld:lifeskim |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:9371698 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:9371698 | pubmed:dateCreated | 1998-1-22 | lld:pubmed |
| pubmed-article:9371698 | pubmed:abstractText | Screening of a human foetal brain genomic DNA library allowed us to isolate an EcoRI-EcoRI fragment containing 6 kb of the 5'-flanking region, the open reading frame and 4 kb of the 3'-flanking region of the alpha2C4 gene. Analysis of the sequenced region (4850 bp) revealed that the first 900 bp 5' to the start codon are very rich in GC (84%), contain several Sp1-binding sites and lack a consensus TATA box. The 5'- and 3'-ends of the alpha2C4 transcript were determined by RNase-protection assays carried out with a series of antisense probes. The data obtained with cellular RNA from HepG2 cells demonstrated that transcription is initiated 891 bases upstream of the translation-start site and that the polyadenylation site is located 550 bases downstream of the stop codon. These results are consistent with the existence of a non-conventional TATA box (TTAGAAA) and the presence of a unique polyadenylation signal (AATAAA). They also fit with the size of alpha2C4-RNA found by Northern-blot analysis (2.9 kb). The transcriptional activity of the alpha2C4 promoter region was investigated by transfecting several cell types with chimaeric constructs containing various fragments of the 5'-non-coding region and luciferase as a reporter gene. The activity of the construct containing the entire 5'-non-coding region appeared to depend on the host cell. Removal of the 5'-untranslated region resulted in loss of cell specificity and a concomitant increase in luciferase activity. Transfection of HepG2 and SK-N-MC cells with constructs deleted of additional 5'-flanking fragments permitted the definition of a minimal 200 bp promoter fragment containing the pseudo-TATA box and two putative SP1-binding sites. | lld:pubmed |
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| pubmed-article:9371698 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9371698 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9371698 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9371698 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9371698 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:9371698 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:9371698 | pubmed:author | pubmed-author:ParisHH | lld:pubmed |
| pubmed-article:9371698 | pubmed:author | pubmed-author:SchaafNN | lld:pubmed |
| pubmed-article:9371698 | pubmed:author | pubmed-author:DevedjianJ... | lld:pubmed |
| pubmed-article:9371698 | pubmed:author | pubmed-author:CaylaCC | lld:pubmed |
| pubmed-article:9371698 | pubmed:author | pubmed-author:SenderYY | lld:pubmed |
| pubmed-article:9371698 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9371698 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:9371698 | pubmed:volume | 328 ( Pt 2) | lld:pubmed |
| pubmed-article:9371698 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9371698 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9371698 | pubmed:pagination | 431-8 | lld:pubmed |
| pubmed-article:9371698 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:9371698 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9371698 | pubmed:articleTitle | Molecular cloning, sequencing and functional study of the promoter region of the human alpha2C4-adrenergic receptor gene. | lld:pubmed |
| pubmed-article:9371698 | pubmed:affiliation | Institut National de la Santé et de la Recherche Médicale U.317, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France. | lld:pubmed |
| pubmed-article:9371698 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9371698 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:152 | entrezgene:pubmed | pubmed-article:9371698 | lld:entrezgene |
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