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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-1-22
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pubmed:abstractText |
Screening of a human foetal brain genomic DNA library allowed us to isolate an EcoRI-EcoRI fragment containing 6 kb of the 5'-flanking region, the open reading frame and 4 kb of the 3'-flanking region of the alpha2C4 gene. Analysis of the sequenced region (4850 bp) revealed that the first 900 bp 5' to the start codon are very rich in GC (84%), contain several Sp1-binding sites and lack a consensus TATA box. The 5'- and 3'-ends of the alpha2C4 transcript were determined by RNase-protection assays carried out with a series of antisense probes. The data obtained with cellular RNA from HepG2 cells demonstrated that transcription is initiated 891 bases upstream of the translation-start site and that the polyadenylation site is located 550 bases downstream of the stop codon. These results are consistent with the existence of a non-conventional TATA box (TTAGAAA) and the presence of a unique polyadenylation signal (AATAAA). They also fit with the size of alpha2C4-RNA found by Northern-blot analysis (2.9 kb). The transcriptional activity of the alpha2C4 promoter region was investigated by transfecting several cell types with chimaeric constructs containing various fragments of the 5'-non-coding region and luciferase as a reporter gene. The activity of the construct containing the entire 5'-non-coding region appeared to depend on the host cell. Removal of the 5'-untranslated region resulted in loss of cell specificity and a concomitant increase in luciferase activity. Transfection of HepG2 and SK-N-MC cells with constructs deleted of additional 5'-flanking fragments permitted the definition of a minimal 200 bp promoter fragment containing the pseudo-TATA box and two putative SP1-binding sites.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-1334095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-1335743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-1385431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-1677644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-1704314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-1848558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2164221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2172770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2568356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2823383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2835476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2842764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-2855960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7654175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7684725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7688069,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7812219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7908287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7912816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7938162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-7978483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8011430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8098045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8138972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8195230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8308019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8382286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8647344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8808178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-8967963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371698-9152409
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
328 ( Pt 2)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
431-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9371698-Base Composition,
pubmed-meshheading:9371698-Base Sequence,
pubmed-meshheading:9371698-Carcinoma, Hepatocellular,
pubmed-meshheading:9371698-Gene Expression,
pubmed-meshheading:9371698-Genes, Reporter,
pubmed-meshheading:9371698-Genomic Library,
pubmed-meshheading:9371698-Humans,
pubmed-meshheading:9371698-Molecular Sequence Data,
pubmed-meshheading:9371698-Promoter Regions, Genetic,
pubmed-meshheading:9371698-RNA, Messenger,
pubmed-meshheading:9371698-RNA, Neoplasm,
pubmed-meshheading:9371698-Receptors, Adrenergic, alpha-2,
pubmed-meshheading:9371698-Recombinant Fusion Proteins,
pubmed-meshheading:9371698-Sequence Analysis, DNA,
pubmed-meshheading:9371698-Transcription, Genetic,
pubmed-meshheading:9371698-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Molecular cloning, sequencing and functional study of the promoter region of the human alpha2C4-adrenergic receptor gene.
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pubmed:affiliation |
Institut National de la Santé et de la Recherche Médicale U.317, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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