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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1997-12-24
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pubmed:abstractText |
The posttranscriptional control element CTE of the simian type D retrovirus has been shown to support replication of Rev-Rev-responsive-element (RRE)-deficient molecular clones of human immunodeficiency virus type 1 (HIV-1). Upon infection of peripheral blood mononuclear cells in vitro, these CTE-containing Rev-independent viruses that are nef+ or nef-minus showed lower replicative capacity and infectivity than the wild-type HIV-1. We studied the effects of Rev-RRE replacement by the CTE on HIV-1 expression with SCID-hu mice. The nef+ and nef-minus Rev-independent viruses established infection with kinetics slower than that of the nef-minus NL4-3. Most importantly, no depletion of CD4-bearing thymocytes was observed after 6 weeks for mice infected with these Rev-independent viruses. This is in contrast to the infection with both wild-type and nef-minus viruses, which led to varying depletion of thymocytes. These data suggest an attenuated phenotype for growth and cytotoxicity of the Rev-independent HIV-1 clones in SCID-hu mice, independent of the presence of Nef. The mutant viruses, which have the essential Rev-RRE regulatory system eliminated, display a distinct phenotype not previously observed with HIV mutant viruses having deletions of accessory genes. Therefore, replacement of the Rev-RRE regulatory axis may generate viruses with altered biological properties in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-1301062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-1741615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-2243384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-2783738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-2981931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-3016298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-3821727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-7666526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-7924427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-7966585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8067053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8100043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8108397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8189487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8294863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8376927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8515816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8627668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8638402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8709222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-8985392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9371653-9191842
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
71
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9817-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9371653-Animals,
pubmed-meshheading:9371653-Antigens, CD4,
pubmed-meshheading:9371653-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9371653-Gene Products, nef,
pubmed-meshheading:9371653-Genes, rev,
pubmed-meshheading:9371653-HIV-1,
pubmed-meshheading:9371653-Humans,
pubmed-meshheading:9371653-Leukocytes, Mononuclear,
pubmed-meshheading:9371653-Lymphocyte Depletion,
pubmed-meshheading:9371653-Mice,
pubmed-meshheading:9371653-Severe Combined Immunodeficiency,
pubmed-meshheading:9371653-Viral Load,
pubmed-meshheading:9371653-Virus Replication,
pubmed-meshheading:9371653-nef Gene Products, Human Immunodeficiency Virus
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pubmed:year |
1997
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pubmed:articleTitle |
Reduced viral load and lack of CD4 depletion in SCID-hu mice infected with Rev-independent clones of human immunodeficiency virus type 1.
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pubmed:affiliation |
ABL-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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