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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-12-12
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pubmed:databankReference |
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pubmed:abstractText |
A Trichoderma reesei cDNA encoding a previously unknown protein with a C-terminal cellulose-binding domain was obtained by complementation screening of a T. reesei cDNA library in a sec1 yeast mutant impaired in protein secretion. The T. reesei protein shows amino acid similarity over its entire length to the Agaricus bisporus cellulose-induced protein CEL1 whose function is not known. These two proteins form a new glycosyl hydrolase family, number 61. Expression of the T. reesei cDNA in yeast showed that it encoded a protein with endoglucanase activity and thus the protein was named EGIV and the corresponding gene egl4. Polyclonal antibodies were prepared against EGIV produced in Escherichia coli and detected a 56-kDa protein in the T. reesei culture supernatant. Northern hybridisation revealed that T. reesei egl4 is regulated in the same manner as other cellulase genes of this fungus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
249
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
584-91
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:9370370-Agaricus,
pubmed-meshheading:9370370-Amino Acid Sequence,
pubmed-meshheading:9370370-Base Sequence,
pubmed-meshheading:9370370-Binding Sites,
pubmed-meshheading:9370370-Cellulase,
pubmed-meshheading:9370370-Cellulose 1,4-beta-Cellobiosidase,
pubmed-meshheading:9370370-Cloning, Molecular,
pubmed-meshheading:9370370-DNA, Complementary,
pubmed-meshheading:9370370-Fungal Proteins,
pubmed-meshheading:9370370-Gene Library,
pubmed-meshheading:9370370-Genes, Fungal,
pubmed-meshheading:9370370-Models, Structural,
pubmed-meshheading:9370370-Molecular Sequence Data,
pubmed-meshheading:9370370-Protein Conformation,
pubmed-meshheading:9370370-Recombinant Proteins,
pubmed-meshheading:9370370-Saccharomyces cerevisiae,
pubmed-meshheading:9370370-Sequence Alignment,
pubmed-meshheading:9370370-Sequence Homology, Amino Acid,
pubmed-meshheading:9370370-Trichoderma
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pubmed:year |
1997
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pubmed:articleTitle |
cDNA cloning of a Trichoderma reesei cellulase and demonstration of endoglucanase activity by expression in yeast.
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pubmed:affiliation |
VTT Biotechnology and Food Research, Espoo, Finland. markku.saloheimo@vtt.fi
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pubmed:publicationType |
Journal Article,
Comparative Study
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