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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1997-12-10
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pubmed:abstractText |
The Src family protein tyrosine kinase Fyn (p59fyn) plays an important role in thymocyte development and T cell receptor (TCR) signal transduction. Fyn has been shown to associate with the TCR-CD3 complex, the protein tyrosine phosphatase CD45 and several co-receptors such as CD28 which are crucial for initiating T cell activation and proliferation. The molecular basis of how Fyn is associated with these transmembrane proteins is largely unknown. To investigate the Fyn association with the TCR-CD3 complex, CD45 and CD28 at the molecular level, various Fyn/beta-galactosidase fusion proteins were constructed and expressed in Jurkat cells. Co-localization experiments applying antibody-induced co-capping and double immunofluorescence staining techniques were used to study the association of these fusion proteins with the TCR-CD3 complex, CD45 and CD28. Our results revealed that co-localization of Fyn with the TCR-CD3 complex requires the unique N terminus whereas co-localization with CD45 depends on the unique N terminus, the Src homology (SH)3- and a functional SH2 domain. CD28 co-localizes with Fyn molecules that contain the N terminus and a functional SH2 domain. These results suggest that Fyn association with the TCR-CD3 complex, CD45 and CD28 is mediated by different molecular mechanisms.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/FYN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fyn,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor-CD3 Complex, Antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2643-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9368621-Antigens, CD28,
pubmed-meshheading:9368621-Antigens, CD3,
pubmed-meshheading:9368621-Antigens, CD45,
pubmed-meshheading:9368621-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:9368621-Humans,
pubmed-meshheading:9368621-Immunologic Capping,
pubmed-meshheading:9368621-Leukemia-Lymphoma, Adult T-Cell,
pubmed-meshheading:9368621-Lymphocyte Activation,
pubmed-meshheading:9368621-Macromolecular Substances,
pubmed-meshheading:9368621-Mutagenesis, Site-Directed,
pubmed-meshheading:9368621-Proto-Oncogene Proteins,
pubmed-meshheading:9368621-Proto-Oncogene Proteins c-fyn,
pubmed-meshheading:9368621-Receptor-CD3 Complex, Antigen, T-Cell,
pubmed-meshheading:9368621-Recombinant Fusion Proteins,
pubmed-meshheading:9368621-Sequence Deletion,
pubmed-meshheading:9368621-Signal Transduction,
pubmed-meshheading:9368621-Transfection,
pubmed-meshheading:9368621-Tumor Cells, Cultured,
pubmed-meshheading:9368621-src Homology Domains
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pubmed:year |
1997
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pubmed:articleTitle |
Co-localization of Fyn with CD3 complex, CD45 or CD28 depends on different mechanisms.
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pubmed:affiliation |
Roche Research Center, Hoffmann-La Roche Inc., Nutley, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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