Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1997-12-24
|
| pubmed:abstractText |
The genes for three small ribonucleases from different strains of Streptomyces aureofaciens have been cloned and expressed in Escherichia coli. The purification of these ribonucleases from the periplasmic space is described. The yields range from 10 to 50 mg of protein per liter of culture medium. The molar absorption coefficients, isoelectric pH values, and pH of optimum activity are reported.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1046-5928
|
| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1997 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
162-8
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9367812-Amino Acid Sequence,
pubmed-meshheading:9367812-Escherichia coli,
pubmed-meshheading:9367812-Gene Expression,
pubmed-meshheading:9367812-Genetic Vectors,
pubmed-meshheading:9367812-Isoenzymes,
pubmed-meshheading:9367812-Molecular Sequence Data,
pubmed-meshheading:9367812-Recombinant Proteins,
pubmed-meshheading:9367812-Ribonucleases,
pubmed-meshheading:9367812-Sequence Homology, Amino Acid,
pubmed-meshheading:9367812-Streptomyces
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Purification of ribonucleases Sa, Sa2, and Sa3 after expression in Escherichia coli.
|
| pubmed:affiliation |
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|