|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1997-12-5
|
|
pubmed:abstractText |
The hok/sok locus of plasmid R1 mediates plasmid stabilization by killing of plasmid-free cells. The locus specifies two RNAs, hok mRNA and Sok antisense RNA. The post-segregational killing mediated by hok/sok is governed by a complicated control mechanism that involves both post-transcriptional inhibition of translation by Sok-RNA and activation of hok translation by mRNA 3' processing. Sok-RNA inhibits translation of a reading frame (mok) that overlaps with hok, and translation of hok is coupled to translation of mok. In the inactive full-length hok mRNA, the translational activator element at the mRNA 5'-end (tac) is sequestered by the fold-back-inhibitory element located at the mRNA 3'-end (fbi). The 5' to 3' pairing locks the RNA in an inert configuration in which the SDmok and Sok-RNA target regions are sequestered. Here we show that the 3' processing leads to major structural rearrangements in the mRNA 5'-end. The structure of the refolded RNA explains activation of translation and antisense RNA binding. The refolded RNA contains an antisense RNA target stem-loop that presents the target nucleotides in a single-stranded conformation. The stem of the target hairpin contains SDmok and AUGmok in a paired configuration. Using toeprinting analysis, we show that this pairing keeps SDmok in an accessible configuration. Furthermore, a mutational analysis shows that an internal loop in the target stem is prerequisite for efficient translation and antisense RNA binding.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hok protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease H,
http://linkedlifedata.com/resource/pubmed/chemical/Sok antisense RNA, E coli
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0022-2836
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
17
|
|
pubmed:volume |
273
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
38-51
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:9367744-Apoptosis,
pubmed-meshheading:9367744-Bacterial Proteins,
pubmed-meshheading:9367744-Bacterial Toxins,
pubmed-meshheading:9367744-Base Sequence,
pubmed-meshheading:9367744-Blotting, Northern,
pubmed-meshheading:9367744-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9367744-Escherichia coli Proteins,
pubmed-meshheading:9367744-Gene Expression Regulation,
pubmed-meshheading:9367744-Molecular Sequence Data,
pubmed-meshheading:9367744-Mutagenesis, Site-Directed,
pubmed-meshheading:9367744-Nucleic Acid Conformation,
pubmed-meshheading:9367744-Nucleic Acid Hybridization,
pubmed-meshheading:9367744-Plasmids,
pubmed-meshheading:9367744-Protein Biosynthesis,
pubmed-meshheading:9367744-RNA,
pubmed-meshheading:9367744-RNA, Antisense,
pubmed-meshheading:9367744-RNA, Bacterial,
pubmed-meshheading:9367744-RNA, Messenger,
pubmed-meshheading:9367744-Ribonuclease H,
pubmed-meshheading:9367744-Sequence Deletion,
pubmed-meshheading:9367744-Transcription, Genetic
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Programmed cell death by hok/sok of plasmid R1: processing at the hok mRNA 3'-end triggers structural rearrangements that allow translation and antisense RNA binding.
|
|
pubmed:affiliation |
Department of Molecular Biology, Odense University, Denmark.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
