Linked Life Data

9367547

Source:http://linkedlifedata.com/resource/pubmed/id/9367547

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-2-4
pubmed:abstractText
Interleukin 6 (IL-6), tumour necrosis factor alpha (TNF-alpha), interleukin 1 (IL-1), and transforming growth factors alpha and beta (TGF-alpha and TGF-beta) are important mediators which play a pleiotropic role in both inflammatory and hepatic regeneration processes. It has also been proposed that a major hepatectomy impairs the liver-related host defence mechanisms. The aim of this study was to evaluate the influence of minor (30%) vs major (80%) hepatectomy on cytokines, growth factors and acute-phase proteins both at the protein and mRNA levels in rat. For that purpose, rats were submitted to either 30% or 80% hepatectomy and sacrificed at intervals up to day 14 post-hepatectomy to collect liver and blood samples. Serum levels of IL-6 and acute-phase proteins (APPs) were determined after RNA extraction, cytokine and acute-phase proteins gene expression were evaluated using a quantitative RT-PCR method. The results demonstrate that liver mRNA levels for IL-6 were early unregulated after a 80% resection only, whereas liver mRNA levels for IL-1 slowly increased following 30 or 80% hepatectomy. For TNF-alpha, no significant changes were observed between groups. Growth factor expression differed according to the extent of hepatic resection. Moreover, plasma levels of alpha2-macroglobulin (alpha2M) and alpha1 acid glycoprotein (AGP), two major APPs which respond differently to combination of cytokines, were significantly lowered after a major resection whereas levels of serum IL-6 showed no significant changes between groups. Paradoxically, in the 80% hepatectomized group, alpha2M mRNA expression was strongly increased at 4 h and 6 h post-hepatectomy as compared with the 30% hepatectomized group. Taken together, these results suggest that, although an increased level of hepatic IL-6 expression was observed following a major resection, the liver's capacity to synthesize normal levels of APPs was impaired. Moreover, these specific changes of cytokine gene expression seen in the liver following major hepatectomy might reflect a preferential activation of the IL-6-dependent APPs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1043-4666
pubmed:author
pubmed:copyrightInfo
Copyright 1997 Academic Press Limited.
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
859-67
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:9367547-Acute-Phase Proteins, pubmed-meshheading:9367547-Animals, pubmed-meshheading:9367547-Cytokines, pubmed-meshheading:9367547-Gene Expression, pubmed-meshheading:9367547-Growth Substances, pubmed-meshheading:9367547-Hepatectomy, pubmed-meshheading:9367547-Interleukin-1, pubmed-meshheading:9367547-Interleukin-6, pubmed-meshheading:9367547-Liver, pubmed-meshheading:9367547-Liver Regeneration, pubmed-meshheading:9367547-Male, pubmed-meshheading:9367547-Polymerase Chain Reaction, pubmed-meshheading:9367547-RNA, Messenger, pubmed-meshheading:9367547-Rats, pubmed-meshheading:9367547-Rats, Sprague-Dawley, pubmed-meshheading:9367547-Transforming Growth Factor alpha, pubmed-meshheading:9367547-Transforming Growth Factor beta, pubmed-meshheading:9367547-Tumor Necrosis Factor-alpha
pubmed:year
1997
pubmed:articleTitle
Cytokine gene expression in liver following minor or major hepatectomy in rat.
pubmed:affiliation
Inserm U78, Boisguillaume, France.
pubmed:publicationType
Journal Article