Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1997-11-25
|
| pubmed:abstractText |
Dendritic cells (DC) form a specialized system for presenting Ag to naive or quiescent T cells and consequently play a central role in the induction of T and B cell immunity. In this study we used DC generated from peripheral progenitors to analyze the effect of IL-10 on the accessory function of human DC. We demonstrate that immature DC, harvested on days 9 to 11 and exposed to IL-10 for the last 2 days of culture, show a strongly reduced capacity to stimulate a CD4+ T cell response in an allogeneic MLR in a dose-dependent manner. In contrast, fully mature DC are completely resistant to the effects of IL-10. These results were obtained in both an alloantigen-induced MLR and an anti-CD3 mAb-induced response of primed and naive (CD45RA+) CD4+ T cells. FACS analysis revealed inhibition of the up-regulation of the costimulatory molecules CD58 and CD86 and the specific DC marker CD83 in DC pretreated with IL-10. These data suggest that IL-10 inhibited the development of fully mature DC. Furthermore, DC precultured with IL-10, but not controls, induced a state of alloantigen-specific anergy in CD4+ T cells and of peptide-specific anergy in the influenza hemagglutinin-specific T cell clone HA1.7. Analysis of the supernatants of these anergic T cells revealed a reduced production of IL-2 and IFN-gamma compared with that in control cells. Collectively, these data suggest that IL-10 converts immature DC into tolerogenic APC, which might be a useful tool in the therapy of patients with autoimmune or allergic diseases.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
159
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4772-80
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9366401-Antibodies, Monoclonal,
pubmed-meshheading:9366401-Antigen Presentation,
pubmed-meshheading:9366401-Antigens, CD3,
pubmed-meshheading:9366401-Antigens, CD45,
pubmed-meshheading:9366401-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9366401-Cell Differentiation,
pubmed-meshheading:9366401-Cells, Cultured,
pubmed-meshheading:9366401-Clonal Anergy,
pubmed-meshheading:9366401-Clone Cells,
pubmed-meshheading:9366401-Dendritic Cells,
pubmed-meshheading:9366401-Dose-Response Relationship, Immunologic,
pubmed-meshheading:9366401-Epitopes, T-Lymphocyte,
pubmed-meshheading:9366401-Growth Inhibitors,
pubmed-meshheading:9366401-HLA-DR Antigens,
pubmed-meshheading:9366401-Humans,
pubmed-meshheading:9366401-Immune Tolerance,
pubmed-meshheading:9366401-Immunosuppressive Agents,
pubmed-meshheading:9366401-Interleukin-10,
pubmed-meshheading:9366401-Isoantigens,
pubmed-meshheading:9366401-Lymphocyte Activation,
pubmed-meshheading:9366401-Up-Regulation
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Induction of tolerance by IL-10-treated dendritic cells.
|
| pubmed:affiliation |
Department of Dermatology, University of Mainz, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|