Linked Life Data

9361351

Source:http://linkedlifedata.com/resource/pubmed/id/9361351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-1-6
pubmed:abstractText
The mRNA abundance of several hepatic glycolytic and gluconeogenic enzymes and blood hormone concentrations were determined in hemorrhagic hypotension-induced rats before and after resuscitation with lactated Ringer's. Northern blot analysis of total liver RNA after 30 min of hemorrhage showed control values for phospho-enolpyruvate carboxykinase and fructose-1,6-bisphosphatase mRNA, but significantly lower values for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF2K/FBPase) as well as 2.5-fold increases in glucose-6-phosphatase (Glu-6-Pase) mRNA. The latter finding is in agreement with the greatly reduced intracellular levels of fructose-6-phosphate and glucose-6-phosphate, and the results are consistent with a rapid activation of hepatic gluconeogenesis by the concomitant decrease in 6PF2K/FBPase and increase in Glu-6-Pase. Blood insulin levels were decreased during hemorrhage and with resuscitation, whereas glucocorticoids were increased 1.5-fold in both cases. Glucagon was unchanged during hemorrhage, but was reduced with resuscitation. Lactated Ringer's resuscitation seemed to affect 6PF2K/FBPase only, which was restored to, and even exceeded, control values. In contrast, Glu-6-Pase mRNA was increased to fourfold control values. The increase in Glu-6-Pase and the decrease in 6PF2K/FBPase mRNA is probably at the level of altered transcriptional rates, because insulin, which plays a dominant role in the regulation of these genes, was decreased during hemorrhage. It remains to be determined what factors are causing further induction of Glu-6-Pase gene after lactated Ringer's resuscitation when hepatic glucose metabolism seems to have reverted to the glycolytic mode.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
385-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:9361351-Animals, pubmed-meshheading:9361351-Blood Glucose, pubmed-meshheading:9361351-Corticosterone, pubmed-meshheading:9361351-Gene Expression, pubmed-meshheading:9361351-Glucagon, pubmed-meshheading:9361351-Gluconeogenesis, pubmed-meshheading:9361351-Glucose-6-Phosphatase, pubmed-meshheading:9361351-Glycolysis, pubmed-meshheading:9361351-Hypotension, pubmed-meshheading:9361351-Insulin, pubmed-meshheading:9361351-Liver, pubmed-meshheading:9361351-Male, pubmed-meshheading:9361351-Phosphofructokinase-2, pubmed-meshheading:9361351-Phosphoric Monoester Hydrolases, pubmed-meshheading:9361351-Phosphotransferases (Alcohol Group Acceptor), pubmed-meshheading:9361351-RNA, Messenger, pubmed-meshheading:9361351-Rats, pubmed-meshheading:9361351-Rats, Sprague-Dawley, pubmed-meshheading:9361351-Resuscitation, pubmed-meshheading:9361351-Shock, Hemorrhagic
pubmed:year
1997
pubmed:articleTitle
Alterations in hepatic 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and glucose-6-phosphatase gene expression after hemorrhagic hypotension and resuscitation.
pubmed:affiliation
Department of Emergency Medicine, University Medical Center, Stony Brook, NY 11794-8191, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't