Linked Life Data

9359904

Source:http://linkedlifedata.com/resource/pubmed/id/9359904

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-1-29
pubmed:abstractText
Four-barrelled ion-sensitive microelectrodes were developed to enable simultaneous measurement of intracellular free Mg2+ and Na+ concentrations ([Mg2+]i, [Na+]i), intracellular pH and the membrane potential. The electrodes were used to investigate pH-induced [Mg2+]i changes in Retzius neurones of the leech Hirudo medicinalis. The application of propionate or CO2/HCO3--buffered bath solutions caused a transient intracellular acidification, an initial [Mg2+]i decrease and a continuous [Na+]i increase. In the presence of CO2/HCO3- this [Na+]i increase was more pronounced and might be the reason for the slow increase in [Mg2+]i following the initial decrease. The withdrawal of propionate or CO2/HCO3--buffered bath solutions caused a transient alkalinization which was accompanied by a slight but significant [Mg2+]i increase, even in the nominal absence of extracellular Mg2+, while [Na+]i returned to its original value. The alkalinization-induced [Mg2+]i increase could be reduced to about 50% by the application of 1-10 microM cyclosporin A, an inhibitor of the mitochondrial permeability transition pore (MTP). Phenylarsine oxide, an MTP activator, caused a [Mg2+]i increase with characteristics similar to those of the alkalinization-induced increase, which could not be attributed to any changes in [Na+]i or pHi. It is concluded that an intracellular alkalinization might induce the release of Mg2+ from intracellular stores.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
435
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
65-73
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Intracellular alkalinization causes Mg2+ release from intracellular binding sites in leech Retzius neurones.
pubmed:affiliation
Institut für Neurobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstrasse 1, D-40225 Düsseldorf, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't