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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1998-1-14
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pubmed:abstractText |
We have used two new dye sets for automated dye-labeled terminator DNA sequencing. One set consists of four, 4,7-dichlororhodamine dyes (d-rhodamines). The second set consists of energy-transfer dyes that use the 5-carboxy-d-rhodamine dyes as acceptor dyes and the 5- or 6-carboxy isomers of 4'-aminomethylfluorescein as the donor dye. Both dye sets utilize a new linker between the dye and the nucleotide, and both provide more even peak heights in terminator sequencing than the dye-terminators consisting of unsubstituted rhodamine dyes. The unsubstituted rhodamine terminators produced electropherograms in which weak G peaks are observed after A peaks and occasionally C peaks. The number of weak G peaks has been reduced or eliminated with the new dye terminators. The general improvement in peak evenness improves accuracy for the automated base-calling software. The improved signal-to-noise ratio of the energy-transfer dye-labeled terminators combined with more even peak heights results in successful sequencing of high molecular weight DNA templates such as bacterial artificial chromosome DNA.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-1598205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-1925562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-2159476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-2347587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-2443975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-2470026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-3713851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-4000959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-7542800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-7603992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-7753809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-7906398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-8596915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-8604350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-8678292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-8688087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-8938436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-8954521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-9206843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9358158-9207029
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4500-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1997
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pubmed:articleTitle |
New dye-labeled terminators for improved DNA sequencing patterns.
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pubmed:affiliation |
PE Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404, USA. rosenbbb@perkin-elmer.com
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pubmed:publicationType |
Journal Article
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