Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1997-12-16
pubmed:abstractText
Nitric oxide (NO), synthesized from L-arginine by NO synthases (NOS), plays an essential role in the regulation of cerebrovascular tone. Adenoviral vectors have been widely used to transfer recombinant genes to different vascular beds. To determine whether the recombinant endothelial NOS (eNOS) gene can be delivered in vivo to the adventitia of cerebral arteries and functionally expressed, a replication-incompetent adenoviral vector encoding eNOS gene (AdCMVNOS) or beta-galactosidase reporter gene (AdCMVLacZ) was injected into canine cerebrospinal fluid (CSF) via the cisterna magna (final viral titer in CSF, 10(9) pfu/ml). Adventitial transgene expression was demonstrated 24 h later by beta-galactosidase histochemistry and quantification, eNOS immunohistochemistry, and Western blot analysis of recombinant eNOS. Electron microscopy immunogold labeling indicated that recombinant eNOS protein was expressed in adventitial fibroblasts. In AdCMVNOS-transduced arteries, basal cGMP production and bradykinin-induced relaxations were significantly augmented when compared with AdCMVLacZ-transduced vessels (P < 0.05). The increased receptor-mediated relaxations and cGMP production were inhibited by eNOS inhibitors. In addition, the increase in cGMP production was reversed in the absence of calcium, suggesting that the increased NO production did not result from inducible NOS expression. The present study demonstrates the successful in vivo transfer and functional expression of recombinant eNOS gene in large cerebral arteries. It also suggests that perivascular eNOS gene delivery via the CSF is a feasible approach that does not require interruption of cerebral blood flow.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-1310445, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-1314587, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-1730576, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-1847633, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-3134520, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7504210, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7509338, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7510430, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7514592, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7526779, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7532305, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7540517, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7543089, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7553780, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7775450, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-7805260, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8063797, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8248193, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8383455, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8446976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8524847, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8573169, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8602187, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8613858, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8614829, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8626455, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8692835, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8726464, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8755640, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8756010, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8784150, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8791446, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8798458, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8827976, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8827977, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8875487, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-8910295, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-9036860, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-9048652, http://linkedlifedata.com/resource/pubmed/commentcorrection/9356490-9099203
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12568-73
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Effects of in vivo adventitial expression of recombinant endothelial nitric oxide synthase gene in cerebral arteries.
pubmed:affiliation
Department of Anesthesiology and Pharmacology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't