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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-12-5
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pubmed:abstractText |
The expression of ciliary neurotrophic factor receptor alpha (CNTFRalpha) was investigated in the developing chick dorsal root ganglion (DRG) using affinity-purified anti-CNTFRalpha antibodies. At thoracic levels, CNTFRalpha-immunoreactivity (CNTFRalpha-IR) was first observed at stage 19 (E3) in cells with neuronal morphology. CNTFRalpha-IR is restricted to the neuronal lineage in the DRG throughout development. CNTFRalpha expression precedes that of neuron-specific beta tubulin, Hu antigen, and Q211 antigen, which are markers expressed in developing sensory neurons. [3H]Thymidine-labeling studies showed the onset of CNTFRalpha expression during terminal mitosis of sensory neuron precursors, making CNTFRalpha the earliest known neuronal marker in the DRG. CNTFRalpha-mediated signal transduction was demonstrated in E7 and E11 DRG neuron cultures by CNTF-induced STAT3 phosphorylation. Although low ligand concentrations (5 pM) elicit STAT3 phosphorylation in E7 and E11 DRG neurons, a survival response is only observed in neurons from E11 DRG. This implicates a complex readout mechanism downstream of STAT3 phosphorylation leading to different cellular responses that depend on the age of the DRG neuron. These results argue against a role of CNTFRalpha ligands in the control of early neuron survival but are compatible with other functions in neurogenesis and sensory neuron development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Ciliary Neurotrophic...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/growth promoting activity receptor
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0012-1606
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1997 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
191
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-13
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9356167-Animals,
pubmed-meshheading:9356167-Antibodies,
pubmed-meshheading:9356167-Cell Division,
pubmed-meshheading:9356167-Cells, Cultured,
pubmed-meshheading:9356167-Chick Embryo,
pubmed-meshheading:9356167-DNA-Binding Proteins,
pubmed-meshheading:9356167-Ganglia, Spinal,
pubmed-meshheading:9356167-Gene Expression Regulation, Developmental,
pubmed-meshheading:9356167-Immunohistochemistry,
pubmed-meshheading:9356167-Neurons,
pubmed-meshheading:9356167-Neurons, Afferent,
pubmed-meshheading:9356167-Phosphorylation,
pubmed-meshheading:9356167-Receptor, Ciliary Neurotrophic Factor,
pubmed-meshheading:9356167-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:9356167-Receptors, Nerve Growth Factor,
pubmed-meshheading:9356167-STAT3 Transcription Factor,
pubmed-meshheading:9356167-Signal Transduction,
pubmed-meshheading:9356167-Spinal Cord,
pubmed-meshheading:9356167-Stem Cells,
pubmed-meshheading:9356167-Thymidine,
pubmed-meshheading:9356167-Trans-Activators
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pubmed:year |
1997
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pubmed:articleTitle |
Onset of CNTFRalpha expression and signal transduction during neurogenesis in chick sensory dorsal root ganglia.
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pubmed:affiliation |
Max-Planck-Institut für Hirnforschung, Deutschordenstrasse 46, Frankfurt, D-60528, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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