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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1997-11-13
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pubmed:abstractText |
Treatment of the Helicobacter pylori vacuolating cytotoxin with very low concentrations of formaldehyde resulted in abrogation of toxic activity in both a HeLa cell vacuolation assay and an in vivo assay of gastric epithelial damage. Detoxification had only a minimal effect on the integrity of the oligomeric or monomeric structure. The toxoid retained the ability to bind to target cells and to induce high-titer neutralizing antibodies after immunization of rabbits. Furthermore, oral immunization of mice with the toxoid resulted in protection against infective challenge with mouse-adapted strains of H. pylori. The sensitivity of the toxin to formaldehyde treatment suggests that a few lysine residues in the protein may be essential for toxic activity and that VacA detoxified in this manner may be a potential candidate for inclusion in a vaccine against H. pylori infection and disease.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-1370047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-1702767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-1901617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-2585121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-571437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-7558333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-7591088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-7592587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-7710054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-7886456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8085372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8163943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8446034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8652579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8666665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8719514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8926088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353041-8957151
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4615-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9353041-Animals,
pubmed-meshheading:9353041-Bacterial Proteins,
pubmed-meshheading:9353041-Bacterial Vaccines,
pubmed-meshheading:9353041-Cytotoxins,
pubmed-meshheading:9353041-Formaldehyde,
pubmed-meshheading:9353041-HeLa Cells,
pubmed-meshheading:9353041-Helicobacter pylori,
pubmed-meshheading:9353041-Humans,
pubmed-meshheading:9353041-Male,
pubmed-meshheading:9353041-Mice,
pubmed-meshheading:9353041-Mice, Inbred BALB C,
pubmed-meshheading:9353041-Rabbits
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pubmed:year |
1997
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pubmed:articleTitle |
Detoxification of the Helicobacter pylori cytotoxin.
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pubmed:affiliation |
IRIS, Chiron-Vaccines, Siena, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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