Linked Life Data

9352915

Source:http://linkedlifedata.com/resource/pubmed/id/9352915

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1997-11-20
pubmed:abstractText
Cell extracts of Sphingomonas herbicidovorans MH grown on (R)-mecoprop contained an enzyme activity that selectively converted (R)-mecoprop to 4-chloro-2-methylphenol, whereas extracts of cells grown on (S)-mecoprop contained an enzyme activity selective for the S enantiomer. Both reactions were dependent on alpha-ketoglutarate and ferrous ions. Besides 4-chloro-2-methylphenol, pyruvate and succinate were detected as products of the reactions. Labeling experiments with (18)O2 revealed that both enzyme activities catalyzed a dioxygenation reaction. One of the oxygen atoms of pyruvate and one of the oxygen atoms of succinate were derived from molecular oxygen. Analysis of cell extracts obtained from cells grown on different substrates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that growth on (R)-mecoprop and (S)-mecoprop caused the appearance of prominent protein bands at 34 and 32 kDa, respectively. Both protein bands were present when cells grew on the racemic mixture. The results demonstrate that S. herbicidovorans initiated the degradation of each enantiomer of mecoprop by a specific alpha-ketoglutarate-dependent dioxygenase. By comparing conversion rates of various phenoxy herbicides, we confirmed that the two enzyme activities were distinct from that of TfdA, which catalyzes the first step in the degradation of 2,4-dichlorophenoxyacetic acid in Ralstonia eutropha JMP134.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-1366457, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-1387319, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-1389314, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-15091913, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-16535134, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-16535265, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-16535583, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-4005444, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-6281585, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-7538273, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-7791906, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-8226980, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-8449871, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-8458850, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-8534119, http://linkedlifedata.com/resource/pubmed/commentcorrection/9352915-8953704
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
179
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6674-9
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Involvement of two alpha-ketoglutarate-dependent dioxygenases in enantioselective degradation of (R)- and (S)-mecoprop by Sphingomonas herbicidovorans MH.
pubmed:affiliation
Swiss Federal Institute for Environmental Science and Technology (EAWAG), Dübendorf, Switzerland.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't