Linked Life Data

9351223

Source:http://linkedlifedata.com/resource/pubmed/id/9351223

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-2-10
pubmed:abstractText
Lactobacillus gasseri 2459, isolated from the human vagina, exhibits a strong autoaggregating phenotype. Filter-sterilized spent supernatants of this strain promote aggregation of Lact. plantarum LL441 and Enterococcus faecalis EF. Aggregation was abolished upon exposure of the cells to proteases and, in the case of Ent. faecalis, to metaperiodate, which suggests the involvement of cell-surface proteins and glycoproteins, respectively, in the aggregation phenotype. In accordance with this, a 75 kDa surface protein, and possibly another of approximately 94 kDa, appears in Lact. plantarum LL441 cultures incubated with Lact. gasseri culture supernatants. The diffusible aggregation promoting factor was purified from stationary phase culture supernatants and determined to be a 2 kDa hydrophilic peptide active at pH 3-4 and stable at neutral and acid pH. The activity was resistant to heat, chymotrypsin, chelating agents, triton X-100 and reducing agents, but sensitive to other proteases and SDS.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1364-5072
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
413-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Characterization of the aggregation promoting factor from Lactobacillus gasseri, a vaginal isolate.
pubmed:affiliation
Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't