Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-2-3
pubmed:abstractText
Seventy-four E. coli strains isolated from piglets with diarrhea or edema disease in Spain were serotyped and examined for production of heat-labile (LT) and heat-stable (ST) enterotoxins (LT-I, LT-II, STaH, STaP, and STb) and verotoxins (VT1, VT2, and VT2v = VTe) by phenotypic (Vero cell assay and infant mouse test) and genotypic (colony hybridization and PCR) methods. In general, an excellent correlation was found between the results obtained with a PCR approach and those determined with biological assays. DNA probes used in the hybridization also showed a very good agreement with phenotypic results, with the exception of a VT1 probe that initially produced 10 false-positive reactions. The gene coding for STb (58 strains) was the most prevalent gene detected by PCR, followed by those coding for STa (46 strains), LT (19 strains), VT2v (11 strains), and VT1 (1 strain). Apparently, in Spain three seropathotypes are predominant: (i) O149:K91:H10 K88+ LT-I+ STb+, (ii) O141:K85ab:H- P987+ STaP+, and (iii) O138:K81:H14 or H- STaP+ VT2v+. We conclude that PCR is a fast, specific, and practical method for the identification of enterotoxin and VT genes in clinical and epidemiological studies.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-1424503, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-1626374, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-1679765, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-1679898, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-1684712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-1791847, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2031306, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2120109, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2182671, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2229362, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2454939, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2842369, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-2869725, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-3055658, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-3290494, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-3511028, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-3513674, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-3526703, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-6352495, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-6657780, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-7045250, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-7814472, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-7886925, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-8057829, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-8231968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-8239131, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-8604549, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-8701567, http://linkedlifedata.com/resource/pubmed/commentcorrection/9350767-881736
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2958-63
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Genes coding for enterotoxins and verotoxins in porcine Escherichia coli strains belonging to different O:K:H serotypes: relationship with toxic phenotypes.
pubmed:affiliation
Departamento de Microbiología y Parasitología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't