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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-12-16
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pubmed:abstractText |
Interleukin-8 possesses chemotactic-activating properties toward neutrophils, and may contribute to the pathogenesis of middle ear inflammation. GRO/CINC-1 is a rat chemokine with structural and functional homology to human interleukin-8, the induction and regulation of which in the middle ear cavity in vivo remains to be established. The production of GRO/CINC-1 in middle ear lavage and gene expression in the middle mucosa was investigated using topical inoculation with lipopolysaccharide (LPS) in the rat in vivo model. GRO/CINC-1 in middle ear lavage showed time- and dose-dependent production under LPS stimulation. The peak of the GRO/CINC-1 production was reached by 4 h after LPS 1 h exposure, whereas the level of production subsequently returned to the level without LPS stimulation at 8 h after LPS stimulation. The topical corticosteroid perfusion in the middle ear after LPS stimulation significantly reduced the production of GRO/CINC-1 in the middle ear cavity compared with that without corticosteroid. At the time of peak production, the expression of GRO/CINC-1 mRNA, evaluated using the polymerase chain reaction, was considerable in the middle ear mucosa. This investigation of the characteristics of interleukin-8-like cytokine in the middle ear cavity using a rat in vivo model has extended the functional concept of chemokines at the initial stage in otitis media.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CXCL1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0001-6489
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
117
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
719-23
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9349869-Animals,
pubmed-meshheading:9349869-Chemokine CXCL1,
pubmed-meshheading:9349869-Chemokines,
pubmed-meshheading:9349869-Chemokines, CXC,
pubmed-meshheading:9349869-Chemotactic Factors,
pubmed-meshheading:9349869-Dose-Response Relationship, Drug,
pubmed-meshheading:9349869-Ear, Middle,
pubmed-meshheading:9349869-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9349869-Gene Expression,
pubmed-meshheading:9349869-Growth Substances,
pubmed-meshheading:9349869-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:9349869-Lipopolysaccharides,
pubmed-meshheading:9349869-Male,
pubmed-meshheading:9349869-Otitis Media,
pubmed-meshheading:9349869-RNA, Messenger,
pubmed-meshheading:9349869-RNA-Directed DNA Polymerase,
pubmed-meshheading:9349869-Rats,
pubmed-meshheading:9349869-Rats, Wistar
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pubmed:year |
1997
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pubmed:articleTitle |
In vivo induction and regulation of interleukin-8-like chemokine GRO/CINC-1 in rat middle ear.
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pubmed:affiliation |
Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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