|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1997-11-24
|
|
pubmed:abstractText |
The HIS7 gene of Saccharomyces cerevisiae encodes a bifunctional glutamine amidotransferase: cyclase that catalyzes the formation of biosynthetic precursors for histidine and adenine. HIS7 is activated by Gcn4p upon amino acid starvation and by the Bas1/2p complex in response to adenine limitation. Mutation analysis of the HIS7 promoter in a deltagcn4 background revealed a polyd(A/T) stretch and a d(CT) repeat as essential elements for Gcn4p-independent basal HIS7 transcription. The protein binding this element was enriched and identified as the multifunctional DNA-binding protein Abf1p. Abf1p binds specifically to the d(CT) repeat sequence, which represents a novel Abf1p-binding motif, and protects 17 nucleotides from digestion by DNase I. In addition, Abf1p binding causes bending of the HIS7 promoter structure.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ABF1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Aminohydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/BAS1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease I,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Poly A,
http://linkedlifedata.com/resource/pubmed/chemical/Poly T,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/imidazole glycerol phosphate...,
http://linkedlifedata.com/resource/pubmed/chemical/poly A-T
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0026-8925
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
256
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
136-46
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:9349705-Aminohydrolases,
pubmed-meshheading:9349705-Cloning, Molecular,
pubmed-meshheading:9349705-DNA, Fungal,
pubmed-meshheading:9349705-DNA-Binding Proteins,
pubmed-meshheading:9349705-Deoxyribonuclease I,
pubmed-meshheading:9349705-Dinucleotide Repeats,
pubmed-meshheading:9349705-Fungal Proteins,
pubmed-meshheading:9349705-Gene Expression Regulation, Fungal,
pubmed-meshheading:9349705-Lac Operon,
pubmed-meshheading:9349705-Multienzyme Complexes,
pubmed-meshheading:9349705-Mutagenesis, Site-Directed,
pubmed-meshheading:9349705-Poly A,
pubmed-meshheading:9349705-Poly T,
pubmed-meshheading:9349705-Polymerase Chain Reaction,
pubmed-meshheading:9349705-Promoter Regions, Genetic,
pubmed-meshheading:9349705-Protein Biosynthesis,
pubmed-meshheading:9349705-Protein Kinases,
pubmed-meshheading:9349705-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9349705-Saccharomyces cerevisiae,
pubmed-meshheading:9349705-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:9349705-Sequence Deletion,
pubmed-meshheading:9349705-Trans-Activators,
pubmed-meshheading:9349705-Transcription, Genetic,
pubmed-meshheading:9349705-Transcription Factors,
pubmed-meshheading:9349705-Transferases,
pubmed-meshheading:9349705-beta-Galactosidase
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Regulation of the yeast HIS7 gene by the global transcription factor Abf1p.
|
|
pubmed:affiliation |
Institut für Mikrobiologie und Genetik Georg-August-Universität, Göttingen, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
