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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1998-1-15
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pubmed:abstractText |
We are reporting the results of HLA-A typing by PCR-SSOP complemented by PCR-SSP of samples obtained from the National Marrow Donor Program (NMDP). These samples were a representative group from 2486 tested in duplicate by serology. A total of 390 samples gave HLA-A discrepant results. Comparing the molecular typing results of 238 samples (samples with available DNA) with the serological typing results, 54 homozygotes and 184 heterozygotes produced a total of 422 assignments by molecular methods. We found assignment discrepancies in 147/422 (35%) in laboratory 1 and 144/422 (34%) in laboratory 2 (a combined group of 4 NMDP laboratories; laboratory 1 is not included). The serological discrepancies found were of 3 categories: a) false negatives, b) incomplete typing (discrepancies due to the level of resolution within a cross-reactive or CREG group) and c) false positives. Major problems were identified using serology for typing HLA-A antigens: a) inability to identify all WHO-recognized specificities, more frequently in non-Caucasians or in HLA-A specificities known to be found more frequently in non-Caucasians for laboratory 1 and incorrect assignments of A19 specificities in laboratory 2, b) incorrect assignments in cells with poor viability and c) false-positive assignments in homozygotes. We propose a possible strategy to type HLA-A specificities with two steps: a) a minimum of serology for typing specificities for common CREG groups: A1, A2, A3, A11, A9, A10, A28, A19. However, a given laboratory can determine the level of serological assignments needed as a first step. And b) molecular methods to identify splits: A23, A24, A29, A30, A31, A32, A33, A34, A36, A66, A74 and A80. The technique described is useful for large-scale bone marrow donor typings for cells with poor viability, and for resolving ambiguous results including false-positive assignments of homozygous cells.
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pubmed:commentsCorrections | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0001-2815
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
380-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9349623-Continental Population Groups,
pubmed-meshheading:9349623-DNA,
pubmed-meshheading:9349623-DNA Probes, HLA,
pubmed-meshheading:9349623-Diagnostic Errors,
pubmed-meshheading:9349623-Evaluation Studies as Topic,
pubmed-meshheading:9349623-Genes, MHC Class I,
pubmed-meshheading:9349623-Genotype,
pubmed-meshheading:9349623-HLA-A Antigens,
pubmed-meshheading:9349623-Histocompatibility Testing,
pubmed-meshheading:9349623-Humans,
pubmed-meshheading:9349623-Polymerase Chain Reaction,
pubmed-meshheading:9349623-Sensitivity and Specificity,
pubmed-meshheading:9349623-Serologic Tests
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pubmed:year |
1997
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pubmed:articleTitle |
Accurate typing of HLA-A antigens and analysis of serological deficiencies.
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pubmed:affiliation |
American Red Cross Blood Services, New England Region, Dedham, Massachusetts 02026, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Comment,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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