Linked Life Data

9349465

Source:http://linkedlifedata.com/resource/pubmed/id/9349465

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:9349465rdf:typepubmed:Citationlld:pubmed
pubmed-article:9349465lifeskim:mentionsumls-concept:C0007634lld:lifeskim
pubmed-article:9349465lifeskim:mentionsumls-concept:C0040300lld:lifeskim
pubmed-article:9349465lifeskim:mentionsumls-concept:C0033684lld:lifeskim
pubmed-article:9349465lifeskim:mentionsumls-concept:C0006023lld:lifeskim
pubmed-article:9349465lifeskim:mentionsumls-concept:C1511790lld:lifeskim
pubmed-article:9349465lifeskim:mentionsumls-concept:C0679622lld:lifeskim
pubmed-article:9349465lifeskim:mentionsumls-concept:C0205314lld:lifeskim
pubmed-article:9349465pubmed:dateCreated1997-11-17lld:pubmed
pubmed-article:9349465pubmed:abstractTextBorna disease (BD) is a transmissible, progressive polioencephalomyelitis primarily of horses and sheep. The genomes of two cell-adapted strains of Borna disease virus (BDV), the aetiological agent of BD, have been cloned and sequenced. According to the structural characterization achieved so far, BDV contains a non-segmented negative-sense 8.9 kb single-stranded RNA genome. In this paper we report the expression, purification and intracellular tracing of a novel non-glycosylated BDV-specific protein with a molecular mass of approximately 10 kDa (BDV p10 protein). The successful isolation of the corresponding mRNA from infected cells, amplification of the genetic region by RT-PCR and its efficient expression as a glutathione S-transferase (GST) fusion protein demonstrated that antibodies specific for the BDV p10 protein are induced in infected animals. In addition, we have produced monospecific antisera against the GST-p10 fusion protein in rabbits. This monospecific antiserum recognized the BDV p10 protein in brain cells of naturally and experimentally infected animals as well as in persistently BDV-infected cells. Antibody-mediated affinity-chromatography using the anti-p10 serum could successfully be applied to purify a ca. 10 kDa antigen from infected animal cells to such an extent that glycosylation of this component could be ruled out.lld:pubmed
pubmed-article:9349465pubmed:languageenglld:pubmed
pubmed-article:9349465pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9349465pubmed:citationSubsetIMlld:pubmed
pubmed-article:9349465pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9349465pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9349465pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9349465pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9349465pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:9349465pubmed:statusMEDLINElld:pubmed
pubmed-article:9349465pubmed:monthOctlld:pubmed
pubmed-article:9349465pubmed:issn0022-1317lld:pubmed
pubmed-article:9349465pubmed:authorpubmed-author:WehnerTTlld:pubmed
pubmed-article:9349465pubmed:authorpubmed-author:BechtHHlld:pubmed
pubmed-article:9349465pubmed:authorpubmed-author:FreseKKlld:pubmed
pubmed-article:9349465pubmed:authorpubmed-author:RichtJ AJAlld:pubmed
pubmed-article:9349465pubmed:authorpubmed-author:RuppertAAlld:pubmed
pubmed-article:9349465pubmed:authorpubmed-author:HerdenCClld:pubmed
pubmed-article:9349465pubmed:issnTypePrintlld:pubmed
pubmed-article:9349465pubmed:volume78 ( Pt 10)lld:pubmed
pubmed-article:9349465pubmed:ownerNLMlld:pubmed
pubmed-article:9349465pubmed:authorsCompleteYlld:pubmed
pubmed-article:9349465pubmed:pagination2459-66lld:pubmed
pubmed-article:9349465pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:meshHeadingpubmed-meshheading:9349465-...lld:pubmed
pubmed-article:9349465pubmed:year1997lld:pubmed
pubmed-article:9349465pubmed:articleTitleDetection of a novel Borna disease virus-encoded 10 kDa protein in infected cells and tissues.lld:pubmed
pubmed-article:9349465pubmed:affiliationInstitut für Virologie, Giessen, Germany.lld:pubmed
pubmed-article:9349465pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9349465pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:9349465lld:pubmed