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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-11-24
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pubmed:databankReference |
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pubmed:abstractText |
A second glutathione reductase (GR) cDNA has been cloned and sequenced from pea (Pisum sativum L. cv. Birte). This new GR cDNA (GOR2) does not encode a pre-protein with a transit peptide and therefore is most likely to represent a cytosolic GR. It is significantly different at the DNA level from the previously cloned chloroplastidial/mitochondrial pea GR (GOR1), but retains the features characteristic of GRs from all sources and has GR activity when expressed in Escherichia coli. GOR2 maps to linkage group 6 on the pea genome map and it seems likely that this is the only locus for this gene. In contrast to GOR1, transcript levels of GOR2 increase in the recovery (post-stress) phases of both drought and chilling by about ten- and three-fold respectively. GOR2 therefore may play a role in the restoration of the post-stress redox state of the cytosolic glutathione pool.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0167-4412
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
641-54
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9349285-Amino Acid Sequence,
pubmed-meshheading:9349285-Base Sequence,
pubmed-meshheading:9349285-Cloning, Molecular,
pubmed-meshheading:9349285-Cold Temperature,
pubmed-meshheading:9349285-Cytosol,
pubmed-meshheading:9349285-DNA, Complementary,
pubmed-meshheading:9349285-DNA, Plant,
pubmed-meshheading:9349285-Desiccation,
pubmed-meshheading:9349285-Escherichia coli,
pubmed-meshheading:9349285-Gene Expression Regulation, Plant,
pubmed-meshheading:9349285-Glutathione Reductase,
pubmed-meshheading:9349285-Molecular Sequence Data,
pubmed-meshheading:9349285-Open Reading Frames,
pubmed-meshheading:9349285-Peas,
pubmed-meshheading:9349285-RNA, Messenger,
pubmed-meshheading:9349285-RNA, Plant,
pubmed-meshheading:9349285-Recombinant Fusion Proteins,
pubmed-meshheading:9349285-Restriction Mapping,
pubmed-meshheading:9349285-Sequence Analysis, DNA,
pubmed-meshheading:9349285-Sequence Homology, Amino Acid,
pubmed-meshheading:9349285-Sequence Homology, Nucleic Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Cloning and characterisation of a cytosolic glutathione reductase cDNA from pea (Pisum sativum L.) and its expression in response to stress.
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pubmed:affiliation |
John Innes Centre, Colney, Norwich, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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