Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1997-12-23
pubmed:abstractText
Automated reticulocyte counting has become an essential instrument of the hematology laboratory. This automatic technique has lead to diminishing labour tasks and to significant improvements in accuracy and precision compared with the manual microscopic methods. In any event, it adds a considerable expense to the laboratory budget. Here, we report the modified method of applying the new mixture of 1 microL of whole blood with 1 mL of reticulocyte reagent, which we evaluated for its accuracy and precision, instead of using the mixture of 3 microL of whole blood with 3 mL of reticulocyte reagent recommended by the company. We demonstrated the accepted accurate and precise results of percentage and absolute number of retculocyte count, low-stained reticulocyte count and its corpuscular indices; the mean reticulocyte corpuscular volume (MCVr), mean reticulocyte corpuscular hemoglobin concentration (CHCMr), and mean reticulocyte hemoglobin content (CHr). These suggested that, for every red cell assessed, the number, the cell volume, hemoglobin content and concentration are accurately and precisely measured by the modified method while the sub-populations of reticulocyte count and distribution width of reticulocyte indices are variable. In conclusion, our results provided the information that 1) the modified method can be used as a routine test and it provides accurate and precise results; 2) with the modified method, two-thirds of the expense spent for reticulocyte reagent can be saved; 3) it should not be used for research purposes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0125-2208
pubmed:author
pubmed:issnType
Print
pubmed:volume
80 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S62-71
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Evaluation of the reticulocyte count portion of technicon H*3 blood analyzer: lowering the test expense by reducing the reticulocyte reagent.
pubmed:affiliation
Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't