Linked Life Data

9344830

Source:http://linkedlifedata.com/resource/pubmed/id/9344830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-12-31
pubmed:abstractText
Cysteinyl-leukotrienes (CysLTs: LTC4, LTD4 and LTE4) are inflammatory mediators which significantly contribute to the airway obstruction in asthma. At least two distinct receptor subtypes exist for cysteinyl-leukotrienes, the CysLT1- and CysLT2-receptor. The purpose of this study was to test whether sheep trachealis muscle is a useful preparation for further characterization of CysLT2-receptors, previously implicated in contraction of human pulmonary veins. Leukotriene C4 and leukotriene D4 evoked contractile responses, leukotriene C4 being significantly more potent than leukotriene D4, whereas leukotriene E4 failed to elicit contractions. The response to leukotriene C4 exhibited tachyphylaxis upon repeated administration. There were no significant effects of epithelial denudation, NO-synthesis inhibition (L-NAME) or cyclooxygenase inhibition (indomethacin) on the responses to cysteinyl-leukotrienes or cholinergic agonists. Neither was responsiveness to different agonists changed by overnight storage. The responses to leukotriene C4 and leukotriene D4 were markedly potentiated when their metabolism was inhibited by S-hexyl glutathione and L-cysteine. The selective CysLT1-antagonist ICI 198,615 had no significant effect on these responses. However, the combined CysLT1- and CysLT2-antagonist BAY u9773 competitively antagonized leukotriene C4 and leukotriene D4 (pA2 values of 7.0 and 6.8 against leukotriene C4 and leukotriene D4, respectively). The findings support that leukotriene C4 and leukotriene D4 act predominantly on CysLT2-receptors in sheep trachea.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1094-5539
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Functional characterization of receptors for cysteinyl-leukotrienes in sheep trachealis muscle.
pubmed:affiliation
Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't