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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1997-12-16
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pubmed:abstractText |
The CD40 ligand expressed on activated T cells plays a pivotal role in B cell proliferation and differentiation. Mutations in the CD40 ligand gene, which alter its expression on the surface of activated T cells, are associated with the X-linked form of Hyper-IgM syndrome (XHIM). A rapid and simple, three-color whole blood flow cytometry procedure was developed for maximal expression and detection of the CD40L on the surface of in vitro activated CD4+ T cells. Approximately 90% of in vitro activated CD4+ T cells obtained from healthy controls expressed the CD40L compared to only 5% of in vitro activated CD4+ T cells obtained from the XHIM patients. The CD40L was expressed on approximately 50% of the in vitro activated CD4+ T cells obtained from the mothers of XHIM patients, consistent with a diagnosis of their carrier status. This is the first report of a whole blood procedure adapted for routine clinical use which is able to detect abnormal CD40L expression in XHIM patients and carriers.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0090-1229
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1997 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
172-81
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9344700-Adult,
pubmed-meshheading:9344700-Antigens, CD3,
pubmed-meshheading:9344700-Antigens, CD40,
pubmed-meshheading:9344700-CD40 Ligand,
pubmed-meshheading:9344700-Carcinogens,
pubmed-meshheading:9344700-Carrier State,
pubmed-meshheading:9344700-Female,
pubmed-meshheading:9344700-Flow Cytometry,
pubmed-meshheading:9344700-Genetic Linkage,
pubmed-meshheading:9344700-Humans,
pubmed-meshheading:9344700-Hypergammaglobulinemia,
pubmed-meshheading:9344700-Immunoglobulin M,
pubmed-meshheading:9344700-Ionomycin,
pubmed-meshheading:9344700-Ionophores,
pubmed-meshheading:9344700-Ligands,
pubmed-meshheading:9344700-Lymphocyte Activation,
pubmed-meshheading:9344700-Membrane Glycoproteins,
pubmed-meshheading:9344700-Syndrome,
pubmed-meshheading:9344700-T-Lymphocytes,
pubmed-meshheading:9344700-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9344700-Up-Regulation,
pubmed-meshheading:9344700-X Chromosome
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pubmed:year |
1997
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pubmed:articleTitle |
Development of a rapid whole blood flow cytometry procedure for the diagnosis of X-linked hyper-IgM syndrome patients and carriers.
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pubmed:affiliation |
Children's Memorial Hospital, Northwestern University, Chicago, Illinois 60614, USA. mogorman@nwu.edu
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pubmed:publicationType |
Journal Article
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