Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-1-30
|
| pubmed:abstractText |
Immunization with naked plasmid DNA effectively induces both humoral and cell-mediated immunity to vaccine antigens and can confer protection against numerous infectious diseases. To explore the potential use of DNA immunization to induce rotavirus-specific immune responses, we used plasmid DNA encoding the VP4 gene of bovine rotavirus (BRV). Intrasmuscular injection of the plasmid encoding the VP4 gene into C57BI/6 mice induced cell-mediated immunity as measured by cytokine production. Although DNA immunization did not induce a detectable BRV-specific antibody response, DNA-immunized animals were primed for antibody production and a cellular immune response. Following viral inoculation, the immunized animals displayed an enhanced number of BRV-specific antibody-secreting cells and cytotoxic activity. The immune response induced by DNA immunization alone or followed by viral inoculation was biased toward IFN-gamma production (Th1-like). CD4+ lymphocytes were the major source of IFN-gamma production in the spleen following DNA immunization. In contrast, a balanced cytokine production was observed in the spleens of animals receiving whole virus. These experiments showed that DNA immunization with a gene encoding the VP4 protein of BRV stimulated a Th1-like immune response in mice, and this bias in the immune response persisted following exposures to whole virus.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Capsid Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/VP4 protein, Rotavirus,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0882-8245
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
117-27
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9344334-Animals,
pubmed-meshheading:9344334-Antibodies, Viral,
pubmed-meshheading:9344334-Capsid,
pubmed-meshheading:9344334-Capsid Proteins,
pubmed-meshheading:9344334-Cattle,
pubmed-meshheading:9344334-Cell Line,
pubmed-meshheading:9344334-Cell Line, Transformed,
pubmed-meshheading:9344334-Cercopithecus aethiops,
pubmed-meshheading:9344334-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:9344334-Interferon-gamma,
pubmed-meshheading:9344334-Mice,
pubmed-meshheading:9344334-Mice, Inbred C57BL,
pubmed-meshheading:9344334-Rotavirus,
pubmed-meshheading:9344334-Spleen,
pubmed-meshheading:9344334-Th1 Cells,
pubmed-meshheading:9344334-Vaccination,
pubmed-meshheading:9344334-Vaccines, DNA,
pubmed-meshheading:9344334-Vaccines, Synthetic,
pubmed-meshheading:9344334-Viral Vaccines
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
DNA immunization with a bovine rotavirus VP4 gene induces a Th1-like immune response in mice.
|
| pubmed:affiliation |
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|