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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1997-10-31
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pubmed:abstractText |
Increased expression of the multidrug resistance gene (MDR-1)-encoded P-glycoprotein (p170[mdr-1]) is a major cause of tumor cell multidrug resistance. p170(mdr-1) functions as a drug-efflux pump to reduce the cellular accumulation of specific drugs. MDA-MB-435 human breast cancer cells that have been transfected with oncogene c-erbB2 complementary DNA (435.eb cells) express high levels of the transmembrane glycoprotein p185(c-erbB2) and exhibit increased resistance to the chemotherapeutic agent paclitaxel via p170(mdr-1)-independent mechanisms. We have recently discovered that the widely used monoclonal antibody C219, which is specific for p170(mdr-1), may cross-react with p185(c-erbB2) in 435.eb cells. In this study, we have investigated the nature of this cross-reactivity.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8874
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1524-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9337349-Amino Acid Sequence,
pubmed-meshheading:9337349-Antibodies, Monoclonal,
pubmed-meshheading:9337349-Breast Neoplasms,
pubmed-meshheading:9337349-Cross Reactions,
pubmed-meshheading:9337349-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9337349-Epitopes,
pubmed-meshheading:9337349-Female,
pubmed-meshheading:9337349-Humans,
pubmed-meshheading:9337349-Immunohistochemistry,
pubmed-meshheading:9337349-Molecular Sequence Data,
pubmed-meshheading:9337349-P-Glycoprotein,
pubmed-meshheading:9337349-Receptor, erbB-2,
pubmed-meshheading:9337349-Recombinant Proteins,
pubmed-meshheading:9337349-Reproducibility of Results,
pubmed-meshheading:9337349-Sequence Alignment,
pubmed-meshheading:9337349-Transfection,
pubmed-meshheading:9337349-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Cross-reactivity of C219 anti-p170(mdr-1) antibody with p185(c-erbB2) in breast cancer cells: cautions on evaluating p170(mdr-1)
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pubmed:affiliation |
Department of Surgical Oncology, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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