Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
1997-11-4
pubmed:abstractText
The recently discovered nucC locus of Serratia marcescens encodes the cryptic prophage genes nucE, nucD, and nucC. NucC is required for expression of the S. marcescens nuclease and functions as a transcriptional activator of the nuclease gene, nucA. NucE and NucD are dispensable for nuclease expression but were proposed to allow for secretion of the nuclease by Escherichia coli. Here, we show (i) that the NucE protein is membrane bound, (ii) that it can complement the lambda S holin, (iii) that it can be triggered by potassium cyanide, (iv) that it is detrimental to cell viability, and (v) that the concomitant expression of nucE and nucD results in cell lysis. Apparently NucE and NucD function as a holin and an endolysin, respectively. This suggests that their roles in nuclease secretion by E. coli are indirect, possibly through directed cell lysis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-1406491, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-2185249, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-6217351, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-6457237, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-7669346, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-7836307, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-8432697, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-8576068, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-8594195, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-8765293, http://linkedlifedata.com/resource/pubmed/commentcorrection/9335307-8878031
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
179
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6522-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
The Serratia marcescens NucE protein functions as a holin in Escherichia coli.
pubmed:affiliation
Institute of Microbiology and Genetics, Vienna Biocenter, University of Vienna, Austria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't