Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1997-11-24
pubmed:abstractText
Nelfinavir mesylate (formerly AG1343) is a potent and selective, nonpeptidic inhibitor of human immunodeficiency virus type 1 (HIV-1) protease that was discovered by protein structure-based design methodologies. We evaluated the antiviral and cytotoxic effects of two-drug combinations of nelfinavir with the clinically approved antiretroviral therapeutics zidovudine (ZDV), lamivudine (3TC), dideoxycytidine (ddC; zalcitabine), stavudine (d4T), didanosine (ddI), indinavir, saquinavir, and ritonavir and a three-drug combination of nelfinavir with ZDV and 3TC against an acute HIV-1 strain RF infection of CEM-SS cells in vitro. Quantitative assessment of drug interaction was evaluated by a universal response surface approach (W. R. Greco, G. Bravo, and J. C. Parsons, Pharm. Rev. 47:331-385, 1995) and by the method of M. N. Prichard and C. Shipman (Antiviral Res. 14:181-206, 1990). Both analytical methods yielded similar results and showed that the two-drug combinations of nelfinavir with the reverse transcriptase inhibitors ZDV, 3TC, ddI, d4T, and ddC and the three-drug combination with ZDV and 3TC resulted in additive to statistically significant synergistic interactions. In a similar manner, the combination of nelfinavir with the three protease inhibitors resulted in additive (ritonavir and saquinavir) to slightly antagonistic (indinavir) interactions. In all combinations, minimal cellular cytotoxicity was observed with any drug alone and in combination. These results suggest that administration of combinations of the appropriate doses of nelfinavir with other currently approved antiretroviral therapeutic agents in vivo may result in enhanced antiviral activity with no associated increase in cellular cytotoxicity.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-11363891, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-1324648, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-1328402, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-1532766, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-1716649, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-2088205, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-2345291, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-3335022, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-3943113, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-7528253, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-7542804, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-7568331, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-7689746, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-7692816, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-7700387, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-8568296, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-8648209, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-8673921, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-8725999, http://linkedlifedata.com/resource/pubmed/commentcorrection/9333041-8834868
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0066-4804
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2159-64
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Activities of the human immunodeficiency virus type 1 (HIV-1) protease inhibitor nelfinavir mesylate in combination with reverse transcriptase and protease inhibitors against acute HIV-1 infection in vitro.
pubmed:affiliation
Agouron Pharmaceuticals, Inc., San Diego, California 92121, USA.
pubmed:publicationType
Journal Article