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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1997-11-19
|
| pubmed:databankReference | |
| pubmed:abstractText |
The human mitochondrial elongation factor Tu (EF-Tu) is nuclear-encoded and functions in the translational apparatus of mitochondria. The complete human EF-Tu cDNA sequence of 1677 base pairs (bp) with a 101 bp 5'-untranslated region, a 1368 bp coding region, and a 207 bp 3'-untranslated region, has been determined and updated. The predicted protein from this cDNA sequence is approximately 49.8 kDa in size and is composed of 455 amino acids (aa) with a putative N-terminal mitochondrial leader sequence of approximately 50 aa residues. The predicted amino acid sequence shows high similarity to other EF-Tu protein sequences from ox, yeast, and bacteria, and also shows limited similarity to human cystolic elongation factor 1 alpha. The complete size of this cDNA (1677 bp) obtained by cloning and sequencing was confirmed by Northern blot analysis, which showed a single transcript (mRNA) of approximately 1.7 kb in human liver. The genomic structure of this EF-Tu gene has been determined for the first time. This gene contains nine introns with a predicted size of approximately 3.6 kilobases (kb) and has been mapped to chromosome 16p11.2. In addition, an intronless pseudogene of approximately 1.7 kb with 92.6% nucleotide sequence similarity to the EF-Tu gene has also been identified and mapped to chromosome 17q11.2.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0378-1119
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
197
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
325-36
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9332382-Amino Acid Sequence,
pubmed-meshheading:9332382-Animals,
pubmed-meshheading:9332382-Base Sequence,
pubmed-meshheading:9332382-Cell Line,
pubmed-meshheading:9332382-Chromosome Mapping,
pubmed-meshheading:9332382-Chromosomes, Human, Pair 16,
pubmed-meshheading:9332382-Chromosomes, Human, Pair 17,
pubmed-meshheading:9332382-Cloning, Molecular,
pubmed-meshheading:9332382-Cricetinae,
pubmed-meshheading:9332382-DNA, Complementary,
pubmed-meshheading:9332382-Genes,
pubmed-meshheading:9332382-Humans,
pubmed-meshheading:9332382-Hybrid Cells,
pubmed-meshheading:9332382-Introns,
pubmed-meshheading:9332382-Liver,
pubmed-meshheading:9332382-Molecular Sequence Data,
pubmed-meshheading:9332382-Peptide Elongation Factor Tu,
pubmed-meshheading:9332382-Pseudogenes,
pubmed-meshheading:9332382-RNA, Messenger,
pubmed-meshheading:9332382-Sequence Analysis, DNA,
pubmed-meshheading:9332382-Sequence Homology, Amino Acid,
pubmed-meshheading:9332382-Sequence Homology, Nucleic Acid
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
The human mitochondrial elongation factor tu (EF-Tu) gene: cDNA sequence, genomic localization, genomic structure, and identification of a pseudogene.
|
| pubmed:affiliation |
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|