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rdf:type |
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lifeskim:mentions |
umls-concept:C0006556,
umls-concept:C0009017,
umls-concept:C0017262,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0185117,
umls-concept:C0679058,
umls-concept:C1412625,
umls-concept:C1547699,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
1-2
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pubmed:dateCreated |
1997-11-19
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pubmed:databankReference |
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pubmed:abstractText |
The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp for a 592-amino acid polypeptide (Mr = 64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
197
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
289-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9332377-Amino Acid Sequence,
pubmed-meshheading:9332377-Animals,
pubmed-meshheading:9332377-Cloning, Molecular,
pubmed-meshheading:9332377-DNA, Complementary,
pubmed-meshheading:9332377-Escherichia coli,
pubmed-meshheading:9332377-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9332377-Hepatectomy,
pubmed-meshheading:9332377-Hydroxymethyl and Formyl Transferases,
pubmed-meshheading:9332377-Liver,
pubmed-meshheading:9332377-Liver Regeneration,
pubmed-meshheading:9332377-Molecular Sequence Data,
pubmed-meshheading:9332377-Nucleotide Deaminases,
pubmed-meshheading:9332377-Phosphoribosylaminoimidazolecarboxamide Formyltransferase,
pubmed-meshheading:9332377-RNA, Messenger,
pubmed-meshheading:9332377-Rats,
pubmed-meshheading:9332377-Recombinant Proteins,
pubmed-meshheading:9332377-Sequence Analysis, DNA,
pubmed-meshheading:9332377-Sequence Homology, Amino Acid,
pubmed-meshheading:9332377-Sequence Homology, Nucleic Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase.
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pubmed:affiliation |
Department of Food Science and Nutrition, Nara Women's University, Japan.
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pubmed:publicationType |
Journal Article
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