| pubmed-article:9332305 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C0376152 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C0007590 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C0546881 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:9332305 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:9332305 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:9332305 | pubmed:dateCreated | 1997-11-19 | lld:pubmed |
| pubmed-article:9332305 | pubmed:abstractText | Investigation of primitive human haemopoietic cell behaviour requires methodologies for monitoring asynchronously activated cells over several generations. We describe a high-resolution procedure for tracking 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE)- labelled human haemopoietic cells through six cell cycles based on the precise halving of their CFSE-fluorescence at each mitosis. Using this approach in combination with DNA or surface antigen staining, we show that the addition of Flt3-ligand (FL) to a cytokine cocktail consisting of Steel factor, IL-3, IL-6 and G-CSF increased the proportion of CD34+ (CD45RA/CD71)-, but not CD34+(CD45RA/CD71)+, human marrow cells initially recruited into division in vitro, shortened the overall cycle time of their progeny, and enhanced the production of a derivative CD34+CD38- population through several (up to four) cell generations. These studies also showed that during the first 4d there was no detectable apoptosis among the progeny of the CD34+(CD45RA/CD71)- cells generated in the presence of this four-cytokine cocktail, regardless of the presence of FL. The availability of a technique for monitoring changes in the properties of individual cells as a function of their mitotic history and under conditions where they are asynchronously recruited to divide provides a new and powerful approach for studies of the regulation of primitive human haemopoietic cell proliferation and differentiation. | lld:pubmed |
| pubmed-article:9332305 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9332305 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9332305 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9332305 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9332305 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:9332305 | pubmed:issn | 0007-1048 | lld:pubmed |
| pubmed-article:9332305 | pubmed:author | pubmed-author:EavesC JCJ | lld:pubmed |
| pubmed-article:9332305 | pubmed:author | pubmed-author:GinsbergS SSS | lld:pubmed |
| pubmed-article:9332305 | pubmed:author | pubmed-author:NordonR ERE | lld:pubmed |
| pubmed-article:9332305 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9332305 | pubmed:volume | 98 | lld:pubmed |
| pubmed-article:9332305 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9332305 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9332305 | pubmed:pagination | 528-39 | lld:pubmed |
| pubmed-article:9332305 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9332305 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9332305 | pubmed:articleTitle | High-resolution cell division tracking demonstrates the FLt3-ligand-dependence of human marrow CD34+CD38- cell production in vitro. | lld:pubmed |
| pubmed-article:9332305 | pubmed:affiliation | Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada. | lld:pubmed |
| pubmed-article:9332305 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9332305 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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