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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-11-19
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pubmed:abstractText |
Investigation of primitive human haemopoietic cell behaviour requires methodologies for monitoring asynchronously activated cells over several generations. We describe a high-resolution procedure for tracking 5- (and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE)- labelled human haemopoietic cells through six cell cycles based on the precise halving of their CFSE-fluorescence at each mitosis. Using this approach in combination with DNA or surface antigen staining, we show that the addition of Flt3-ligand (FL) to a cytokine cocktail consisting of Steel factor, IL-3, IL-6 and G-CSF increased the proportion of CD34+ (CD45RA/CD71)-, but not CD34+(CD45RA/CD71)+, human marrow cells initially recruited into division in vitro, shortened the overall cycle time of their progeny, and enhanced the production of a derivative CD34+CD38- population through several (up to four) cell generations. These studies also showed that during the first 4d there was no detectable apoptosis among the progeny of the CD34+(CD45RA/CD71)- cells generated in the presence of this four-cytokine cocktail, regardless of the presence of FL. The availability of a technique for monitoring changes in the properties of individual cells as a function of their mitotic history and under conditions where they are asynchronously recruited to divide provides a new and powerful approach for studies of the regulation of primitive human haemopoietic cell proliferation and differentiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5-(6)-carboxyfluorescein diacetate...,
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NAD Nucleosidase,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides,
http://linkedlifedata.com/resource/pubmed/chemical/flt3 ligand protein
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0007-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
528-39
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9332305-ADP-ribosyl Cyclase,
pubmed-meshheading:9332305-Antigens, CD,
pubmed-meshheading:9332305-Antigens, CD34,
pubmed-meshheading:9332305-Antigens, CD38,
pubmed-meshheading:9332305-Antigens, Differentiation,
pubmed-meshheading:9332305-Cell Division,
pubmed-meshheading:9332305-Cell Line,
pubmed-meshheading:9332305-Cell Lineage,
pubmed-meshheading:9332305-Fluoresceins,
pubmed-meshheading:9332305-Fluorescence,
pubmed-meshheading:9332305-Fluorescent Dyes,
pubmed-meshheading:9332305-Growth Substances,
pubmed-meshheading:9332305-Hematopoietic Stem Cells,
pubmed-meshheading:9332305-Humans,
pubmed-meshheading:9332305-Membrane Glycoproteins,
pubmed-meshheading:9332305-Membrane Proteins,
pubmed-meshheading:9332305-NAD+ Nucleosidase,
pubmed-meshheading:9332305-Phenotype,
pubmed-meshheading:9332305-Succinimides
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pubmed:year |
1997
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pubmed:articleTitle |
High-resolution cell division tracking demonstrates the FLt3-ligand-dependence of human marrow CD34+CD38- cell production in vitro.
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pubmed:affiliation |
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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