Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1997-11-24
pubmed:databankReference
pubmed:abstractText
Genetic studies in chickens and receptor interference experiments have indicated that avian leukosis virus (ALV)-E may utilize a cellular receptor related to the receptor for ALV-B and ALV-D. Recently, we cloned CAR1, a tumor necrosis factor receptor (TNFR)-related protein, that serves as a cellular receptor for ALV-B and ALV-D. To determine whether the cellular receptor for ALV-E is a CAR1-like protein, a cDNA library was made from turkey embryo fibroblasts (TEFs), which are susceptible to ALV-E infection, but not to infection by ALV-B and ALV-D. The cDNA library was screened with a radioactively labeled CAR1 cDNA probe, and clones that hybridized with the probe were isolated. A 2.3-kb cDNA clone was identified that conferred susceptibility to ALV-E infection, but not to ALV-B infection, when expressed in transfected human 293 cells. The functional cDNA clone is predicted to encode a 368 amino acid protein with significant amino acid similarity to CAR1. Like CAR1, the TEF protein is predicted to have two extracellular TNFR-like cysteine-rich domains and a putative death domain similar to those of TNFR I and Fas. Flow cytometric analysis and immunoprecipitation experiments demonstrated specific binding between the TEF CAR1-related protein and an immunoadhesin composed of the surface (SU) envelope protein of subgroup E (RAV-0) virus fused to the constant region of a rabbit immunoglobulin. These two activities of the TEF CAR1-related protein, specific binding to ALV-E SU and permitting entry only of ALV-E, have unambiguously identified this protein as a cellular receptor specific for subgroup E ALV.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-171832, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-1731342, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-3009025, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-3714490, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-4350221, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-6245246, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-6270346, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-7769686, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-7815478, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8383211, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8387891, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8397073, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8402880, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8455633, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8523543, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8777713, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8892869, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8945512, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-8970739, http://linkedlifedata.com/resource/pubmed/commentcorrection/9326659-9082980
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
94
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11617-22
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9326659-Alkaline Phosphatase, pubmed-meshheading:9326659-Amino Acid Sequence, pubmed-meshheading:9326659-Animals, pubmed-meshheading:9326659-Arginase, pubmed-meshheading:9326659-Avian Proteins, pubmed-meshheading:9326659-Avian leukosis virus, pubmed-meshheading:9326659-Base Sequence, pubmed-meshheading:9326659-Cell Line, pubmed-meshheading:9326659-Cloning, Molecular, pubmed-meshheading:9326659-Embryo, Nonmammalian, pubmed-meshheading:9326659-Fibroblasts, pubmed-meshheading:9326659-Fungal Proteins, pubmed-meshheading:9326659-Gene Library, pubmed-meshheading:9326659-Humans, pubmed-meshheading:9326659-Membrane Proteins, pubmed-meshheading:9326659-Molecular Sequence Data, pubmed-meshheading:9326659-Rabbits, pubmed-meshheading:9326659-Receptors, Cell Surface, pubmed-meshheading:9326659-Receptors, Virus, pubmed-meshheading:9326659-Recombinant Fusion Proteins, pubmed-meshheading:9326659-Sequence Alignment, pubmed-meshheading:9326659-Sequence Homology, Amino Acid, pubmed-meshheading:9326659-Transfection, pubmed-meshheading:9326659-Turkeys
pubmed:year
1997
pubmed:articleTitle
Identification of a cellular receptor for subgroup E avian leukosis virus.
pubmed:affiliation
Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't